Methods and compositions for the enzymatic production of pseudouridine triphosphate
AssigneeNatures's Toolbox, Inc.
InventorsAlexander Koglin, Michael Humbert, Matthias Strieker, Grant Eliason
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Abstract
The present invention includes novel systems, methods, and compositions for the enzymatic/chemical production of pseudouridine (ψ) and its variants, such as N1-methyl-pseudouridine-5′-triphosphate (m1ψTP).
Claims30
A method producing pseudouridine, the method comprising:
- protecting a pseudouridine-5′-monophosphate (ψMP) with a protecting agent that reacts with the hydroxyl groups of said ψMP forming a protected ψMP;
- methylating the protected ψMP with a methylating agent forming a protected N1-methyl-pseudouridine-5′-monophosphate (protected m1ψMP);
- deprotecting the protected m1ψMP with a deprotection agent forming a N1-methyl-pseudouridine-5′-monophosphate (m1ψMP);
- and
- sequentially phosphorylating the m1ψMP with a phosphorylating agent forming N1-methyl-pseudouridine-5′-diphosphate (m1ψDP) and N1-methyl-pseudouridine-5′-triphosphate (m1ψTP).
A method for the production of a pseudouridine intermediate compound, the method comprising protecting a pseudouridine-5′-monophosphate (ψMP) with a protecting agent that reacts with the hydroxyl groups of said ψMP forming the protected ψMP.
A method for the production of a pseudouridine intermediate compound, the method comprising methylating a protected ψMP with a methylating agent, forming a protected N1-methyl-pseudouridine-5′-monophosphate (protected m1ψMP).
A method for the production of a pseudouridine intermediate compound, the method comprising deprotecting a protected m1ψMP with a deprotection agent forming a N1-methyl-pseudouridine-5′-monophosphate (m1ψMP).
Dependencies are inferred from the claim text.