Production of lytic phages
AssigneeEligo Bioscience
InventorsJesus FERNANDEZ RODRIGUEZ, Antoine DECRULLE, Aymeric LEVEAU, Ines CANADAS BLASCO, Aurelie MATHIEU, Thibault CARLIER
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Abstract
The present invention concerns a production bacterial cell for producing lytic phage particles or lytic phage-derived delivery vehicles, said production bacterial cell stably comprising at least one phage structural genes and at least one phage DNA packaging genes, said phage structural gene(s) and phage DNA packaging gene(s) being derived from a lytic bacteriophage, wherein the expression of at least one of said phage structural genes and/or at least one of said phage DNA packaging gene(s) in said production bacterial cell is controlled by an induction mechanism.
Claims20
Production bacterial cell for producing lytic phage particles or lytic phage-derived delivery vehicles, said production bacterial cell stably comprising at least one phage structural gene and at least one phage DNA packaging gene, said phage structural gene(s) and phage DNA packaging gene(s) being derived from a lytic bacteriophage,
- wherein the expression of at least one of said phage structural genes and/or at least one of said phage DNA packaging gene(s) in said production bacterial cell is controlled by an induction mechanism.
A method for producing lytic phage particles or lytic phage-derived delivery vehicles, comprising:
- (a) providing a production bacterial cell for producing lytic phage particles or lytic phage-derived delivery vehicles, said production bacterial cell stably comprising at least one phage structural gene and at least one phage DNA packaging gene, said phage structural gene(s) and phage DNA packaging gene(s) being derived from a lytic bacteriophage,
- wherein the expression of at least one of said phage structural genes and/or at least one of said phage DNA packaging gene(s) in said production bacterial cell is controlled by an induction mechanism, and
- (b) inducing, in said production bacterial cell, expression of said at least one of said phage structural gene(s) and said at least one of said phage DNA packaging gene(s), and assembly of the products expressed by said at least one phage structural gene(s) and said at least one phage DNA packaging gene(s), thereby producing lytic phage particles or lytic phage-derived delivery vehicles.
Hybrid helper phage system comprising:
- (i) at least one phage DNA packaging gene(s) derived from a lytic bacteriophage,
- (i′) at least one phage structural gene(s) derived from said lytic bacteriophage,
- (i″) optionally, at least one phage gene(s) involved in phage regulation derived from said lytic bacteriophage, and
- (ii) at least one gene, derived from a non-lytic bacteriophage, involved in phage excision/insertion, phage DNA replication, and/or phage regulation,
- wherein said genes (i), (i′), (i″) and (ii) are comprised in a unique nucleic acid molecule or in separate nucleic acid molecules, and
- wherein said hybrid helper phage system does not comprise any expressed phage structural gene derived from said non-lytic bacteriophage.
Dependencies are inferred from the claim text.