Material and method for preparation of small, single-stranded dna circles
AssigneePurdue Research Foundation
InventorsChengde Mao, Victoria Elizabeth Paluzzi, Cuizheng Zhang
Life of this patent
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Abstract
A splint single-stranded DNA (ssDNA), which comprises a linear splint sequence and a hairpin sequence on each side of the linear splint sequence; a splint ssDNA-ligating linear ssDNA monomeric complex; a method of producing a ssDNA circle; and a kit comprising the splint ssDNA and instructions for using the splint ssDNA to produce a ssDNA circle.
Claims21
A splint single-stranded DNA (ssDNA), which comprises a linear splint sequence and a hairpin sequence on each side of the linear splint sequence.
The method of 12, wherein the ratio of ligating linear ssDNA to splint ssDNA is from about 1:1 to about 1:1.5.
Dependencies are inferred from the claim text.