US 2024/0240209 A1 · SYNTHESIS OF ENANTIOPURE CIS-A-IRONE FROM A RENEWABLE CARBON SOURCE

Synthesis of enantiopure cis-a-irone from a renewable carbon source

AssigneesAgency for Science, Technology and Research, Institut National De Recherche Pour L' Agriculture, L'Alimentation Et L'Environment, Centre National De La Recherche Scientifique, Institut National Des Sciences Appliquees De Toulouse

InventorsXixian CHEN, Congqiang ZHANG, Rehka T, Sudha SHUKAL, Derek SMITH, Isabelle ANDRÉ, Esque JEREMY

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Abstract

Synthesis of enantiopure cis-α-irone from a renewable carbon source Disclosed herein are natural and synthetic enzymes capable of performing a method of producing cis-α-irone. The method comprises providing an enzyme capable of converting psi-ionone to cis-α-irone; and contacting the enzyme and psi-ionone under suitable conditions to produce cis-α-irone. The enzyme may be a methyltransferase from Streptomyces albireticuli (SaMT), a promiscuous bifunctional methyltransferase/cyclase (pMT1) enzyme from Streptomyces, or a modified pMT1 enzyme with at least one substitution. The enzymes allow the in-vivo and in-vitro production of cis-α-irone including the use of glucose as feedstock for the biotransformation into cis-α-irone.

Claims46

Dependent claims

A method of producing cis-α-irone, the method comprises providing an enzyme capable of converting psi-ionone to cis-α-irone;

and contacting the enzyme and psi-ionone under suitable conditions to produce cis-α-irone.

A modified enzyme comprising a first substitution of base SEQ ID No. 3 at a position selected from the group consisting of position 200, position 180, position 160, and position 236,

wherein if the first substitution is at position 200, the first substitution is selected from the group consisting of phenylalanine, isoleucine, leucine, valine, and tryptophan,
wherein if the first substitution is at position 180, the first substitution is selected from the group consisting of alanine, cysteine, glutamic acid, isoleucine, methionine, and valine,
wherein if the first substitution is at position 160, the first substitution is selected from the group consisting of alanine, cysteine, histidine, isoleucine, leucine, methionine, asparagine, glutamine, threonine, valine, and tyrosine,
wherein if the first substitution at position 236 is selected, the first substitution is selected from the group consisting of cysteine, glutamic acid, histidine, isoleucine, leucine, asparagine, serine, threonine, and valine.

1 more element

Dependencies are inferred from the claim text.