Universal sensing system
AssigneeAbbott Diabetes Care Inc.
InventorsJosie Corby, Barry S. Kreutz, Hao Lei, Anthony S. Muerhoff, Christopher Marohnic, Thomas P. Leary
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Abstract
Provided herein are systems and methods for the detection and analysis of biomolecules. In particular, provided herein are sensor systems employing an enzyme that comprises an allosteric site that interacts with an inhibitor to determine the presence of, absence of, or amount of one or more analytes of interest in a sample. In some embodiments, the allosteric site comprises a grafted epitope that corresponds to one or more analytes of interest.
Claims21
-123. (canceled)
An enzyme comprising an epitope-grafted allosteric site that is inhibited by contact with an inhibitor and is de-inhibited in the presence of an analyte that binds to said inhibitor.
The system of claim 135, wherein the inhibitor binds to said analyte with a greater affinity than the inhibitor binds to said epitope-grated sequence.
The system of claim 139, wherein said sensor is an electrochemical sensor.
A method of detecting analyte, comprising:
- a) contacting a sample suspected of containing an analyte to an enzyme of claim 1;
- and b) detecting, directly or indirectly, activity of said enzyme.
Dependencies are inferred from the claim text.