Methods for analyzing nucleic acid sequences
AssigneeSynthego Corporation
InventorsTimothy Hsiau, Richard Stoner, Travis Maures, David Conant
Life of this patent
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Abstract
The present disclosure provides a method for analyzing nucleic acid sequences. The method can comprise determining, by a computer system, a base trace by trimming a Sanger sequencing trace of a plurality of nucleic acid molecules from a sample based on a first target sequence and a second target sequence. Each of the first and second target sequences can be in the plurality of nucleic acid molecules or can be in the complement of sequence of the plurality of nucleic acid molecules.
Claims25
1-215. (canceled)
A non-transitory computer readable medium containing computer executable instructions that, when executed by a processor, cause the processor to perform a method comprising:
- determining a plurality of predicted sequences of individual nucleic acid molecules in a sample,
- wherein the sample has been contacted by at least two different nucleic acid editing tools, and
- wherein the determining is based on a Sanger sequencing trace of a plurality of nucleic acid molecules from the sample that has been contacted by the at least two different nucleic acid editing tools;
- assessing an outcome of contacting the sample with the at least two different nucleic acid tools based on determining the plurality of predicted sequences;
- and reporting a predicted knockout sequence to a user.
A non-transitory computer readable medium containing computer executable instructions that, when executed by a processor, cause the processor to perform a method comprising:
- determining a predicted knockout sequence of a single nucleic acid molecule based on a first Sanger sequencing trace and a second Sanger sequencing trace,
- wherein the predicted knockout sequence comprises an indel when compared to the second Sanger sequencing trace;
- and reporting the predicted knockout sequence to a user.
A non-transitory computer readable medium containing computer executable instructions that, when executed by a processor, cause the processor to perform a method comprising:
- determining a first predicted sequence of a single nucleic acid molecule and a second predicted sequence of a single nucleic acid molecule,
- wherein the first and second predicted sequences are based on a first Sanger sequencing trace of a first plurality of nucleic acid molecules from a first sample,
- wherein the first and second predicted sequences each comprises an indel when compared to a second Sanger sequencing trace of a second plurality of nucleic acid molecules from a second sample, and
- wherein the indels of the first and second predicted sequences have a same size and a different nucleic acid sequence;
- and reporting the first predicted sequence and the second predicted sequence to a user.
Dependencies are inferred from the claim text.