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AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJianghong RAO, Tingting DAI, Jinghang XIE, Ran MU
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Caged luciferin-based probes become a luciferase substrate emitting bioluminescence upon β-lactamase/esterase activation. The inclusion of a cephalosporin moiety renders the probe capable of being used for the detection of a wide-range of β-lactamases and β-lactamase-expressing bacteria. Embodiments of a rapid high-throughput assay for the identification of β-lactamase-expressing bacteria is made possible by the use of such probes. In some embodiments the cephalosporin is substituted by a carbapenem moiety to generate carbapenem-caged luciferin carbapenem-cleavable probes capable of being used for the detection of a wide-range of carbapenem-expressing bacteria. Accordingly embodiments of a rapid high-throughput assay for the identification of carbapenem-expressing bacteria is made possible by the use of these probes.
A caged bioluminescent probe comprising a luciferin moiety, an enzyme-cleavable moiety, and a caging moiety, wherein the moieties are conjugated to form the structure having the formula:
A method of identifying a bacterial strain resistant to a β-lactam antibiotic or a carbapenem antibiotic, wherein said method comprises contacting a population of bacteria with a caged bioluminescent probe cleavable by either a β-lactamase or a carbapenemase. adding luciferinase;
Dependencies are inferred from the claim text.