Biosynthetic production of steviol glycosides and processes therefore
AssigneeConagen Inc.
InventorsGuohong Mao, Jacob Edward Vick, Michael Batten, David Byun, Yang Luo, Yilin Wu, Beihua Zhang, Xiaodan Yu
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Abstract
The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.
Claims14
-12. (canceled)
A CP1 recombinant polypeptide encoded by a DNA sequence having at least 80% identity to SEQ ID NO:3.
-38. (canceled)
A steviol glycoside Reb WB1 having the structure:
-46. (canceled)
A method of producing rebaudioside M, comprising cultivating a recombinant cell under suitable growth conditions where said recombinant cell exhibits the ability to produce steviol glycosides, the method comprising:
- contacting said recombinant cell with a reaction composition containing stevioside, sucrose synthase and sucrose;
- wherein said recombinant cell expresses a first UDP-glycosyltransferase (UGT) or a catalytically active portion thereof capable of using said stevioside substrate to produce rebaudioside E;
- wherein said recombinant cell expresses a second UDP-glycosyltransferase (UGT) or a catalytically active portion thereof capable of using said Rebaudioside E to produce rebaudioside D4;
- and,
- wherein said recombinant cell expresses a third UDP-glycosyltransferase (UGT) or a catalytically active portion thereof capable of using said Rebaudioside D4 to produce rebaudioside M.
-54. (canceled)
Dependencies are inferred from the claim text.