Sequential Fermentative Production Of Oligosaccharides
AssigneeChr. Hansen A/S
InventorsKatja Parschat, Stefan Jennewein
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Abstract
Disclosed is a process for the production of a desired oligosaccharide, the process comprises providing a genetically engineered microbial cell which possesses a saccharide importer for the uptake of an intermediate oligosaccharide, and an enzyme being able to convert the intermediated oligosaccharide by transferring a monosaccharide moiety from a donor substrate to the intermediate oligosaccharide; cultivating the genetically engineered microbial cell in the presence of an intermediate oligosaccharide to generate the desired oligosaccharide; and retrieving the desired oligosaccharide.
Claims17
A process for the production of a desired oligosaccharide comprising more than three monosaccharide moieties, wherein the process comprises
- providing a genetically engineered microbial cell that possesses
- a saccharide importer for the uptake of an intermediate oligosaccharide consisting of at least three monosaccharide moieties but less monosaccharide moieties than the desired oligosaccharide by the microbial cell, and
- an enzyme which is able to transfer a monosaccharide moiety from a donor substrate to the intermediate oligosaccharide;
- cultivating the genetically engineered microbial cell in a culture medium which contains the intermediate oligosaccharide for the genetically engineered microbial cell to take up the intermediate oligosaccharide and to transfer at least one monosaccharide moiety to the intermediate oligosaccharide, thereby synthesizing the desired oligosaccharide;
- and
- retrieving the desired oligosaccharide from the culture medium and/or the genetically engineered microbial cell.
(canceled)
A process for the production of lacto-N-neotetraose, wherein the process comprises
- providing a genetically engineered microbial cell that possesses
- a) a transporter which facilitates uptake of LNT-II, and
- b) a β-1,4 galactosyltransferase;
- cultivating the genetically engineered microbial cell in a culture medium which contains LNT-II for the genetically engineered microbial cell to take up LNT-II and to transfer a galactose moiety from UDP-galactose to LNT-II by said β-1,4 galactosyltransferase, thereby synthesizing LNnT;
- and
- retrieving the LNnT from the culture medium and/or the genetically engineered microbial cell.
Dependencies are inferred from the claim text.