Resistant-isomalto-oligosaccharide (imo-r)
OwnerMohammad Hassan QURESHIinventor
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Abstract
A novel protocol is presented for the production of resistant-isomalto-oligosaccharides (“IMO-R”) that are resistant to human gut enzymes, in particular, dextranase, α-glucosidase and stomach and pancreatic α-amylases, thus exhibiting an increasing dietary Fiber effect.
Claims52
A method for preparing a resistant-isomalto-oligosaccharide (“IMO-R”) with increasing resistance to human gut enzymes, the method comprising:
- a) adjusting the pH of a solution of liquified starch to about 4.5 to 5.5;
- b) then adding an effective amount of Fungamyle 800L to the solution;
- c) then incubating the solution at about 50 to 55 degrees Celsius for about 30 minutes, during which the solution is shaken or stirred;
- d) then terminating activity of the Fungamyle 800L by raising the temperature of the solution to that of boiling water for about 30 minutes;
- e) then cooling the solution to room temperature;
- f) then adjusting the pH of the solution to about 5.0 to 5.5;
- g) then adding an effective amount of Transglucosidase (“TG”) to the solution;
- h) then incubating the solution at about 55 to 60 degrees Celsius for about 24 hours, during which the solution is shaken or stirred;
- and
- i) then terminating activity of the TG by raising the temperature of the solution to that of boiling water for about 30 minutes.
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A human gut enzyme resistant isomalto-oligosaccharide (“IMO-R”), as produced by a method comprising:
- a) adjusting the pH of a solution of liquified starch to about 4.5 to 5.5;
- b) then adding an effective amount of Fungamyle 800L to the solution;
- c) then incubating the solution at about 50 to 55 degrees Celsius for about 30 minutes, during which the solution is shaken or stirred;
- d) then terminating activity of the Fungamyle 800L by raising the temperature of the solution to that of boiling water for about 30 minutes;
- e) then cooling the solution to room temperature;
- f) then adjusting the pH of the solution to about 5.0 to 5.5;
- g) then adding an effective amount of Transglucosidase (“TG”) to the solution;
- h) then incubating the solution at about 55 to 60 degrees Celsius for about 24 hours, during which the solution is shaken or stirred;
- and
- i) then terminating activity of the TG by raising the temperature of the solution to that of boiling water for about 30 minutes.
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Dependencies are inferred from the claim text.