Back to results
US 2024/0229090 A1Published application
Modified bacteria for production of nitroaromatics
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsYousong Ding, Ran Zuo
Life of this patent
Estimating the term…
Abstract
The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.
Claims16
1-26. (canceled)
A method of producing a recombinant bacterial cell comprising
- (i) transforming a bacterial cell with:
- (a) an isolated nucleic acid engineered to express a fusion protein comprising a TxtE enzyme linked to a catalytic domain of a CYP102A1 (P450BM3) reductase enzyme via an amino acid linker sequence that is between 14 and 27 amino acids in length;
- (b) an isolated nucleic acid engineered to express a nitric oxide synthase (NOS) enzyme;
- and, optionally
- (c) an isolated nucleic acid engineered to express a glucose dehydrogenase (GDH) enzyme;
- and,
- (ii) culturing the bacterial cell in a bacterial culture media.
4 more elements
29-39. (canceled)
Dependencies are inferred from the claim text.