Apparatus and method for collection of sperm samples
AssigneesTexas Tech University System, Rsi Technology Group, LLC
InventorsLindsay L. PENROSE, Samuel D. PRIEN
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Abstract
The present invention relates to a method and apparatus for passive collection of sperm having improved motility from a semen sample. The semen sample is contacted with on side of a porous barrier and a nutrient-containing media is contacted with the other side of the porous barrier. More robust sperm migrate from the semen sample through the porous barrier and are collected in a nutrient-containing media.
Claims41
An apparatus for passively isolating a population of motile sperm from a semen sample solution, the apparatus comprising:
- a) a first vessel for receiving the semen sample solution, and
- b) a sperm isolation device (SID) for receiving a nutrient media, the device comprising a frame and a porous barrier, the porous barrier comprising a plurality of holes, wherein each hole has a passthrough dimension in a range of from 6 microns to 30 microns.
A method for passively isolating a population of motile sperm from a semen sample solution, the method comprising:
- (i) introducing the semen sample solution into a first vessel;
- (ii) introducing a harvest media into a sperm isolation device (SID) comprising a porous barrier;
- and
- (iii) inserting the SID in the semen sample solution to submerge at least a portion of the porous barrier;
- wherein:
- the porous barrier comprises a plurality of holes, each having a passthrough dimension in a range of from 6 microns to 30 microns;
- and
- a population of motile sperm in the semen sample solution migrate through the holes in the porous barrier into the harvest media.
A method for passively isolating a population of motile sperm from a semen sample solution, the method comprising:
- (i) introducing a harvest media into a first vessel;
- (ii) introducing the semen sample solution into a sperm isolation device (SID) comprising a porous barrier;
- and
- (iii) inserting the SID in the harvest media to submerge at least a portion of the porous barrier;
- wherein:
- the porous barrier comprises a plurality of holes, each having a passthrough dimension in a range of from 6 microns to 30 microns;
- and
- a population of motile sperm in the semen sample solution migrate through the holes in the porous barrier into the harvest media.
A sperm isolation device (SID) for passively isolating a population of motile sperm from a semen sample solution, the device comprising:
- a) a frame;
- and
- b) a porous barrier comprising a plurality of holes, each having a passthrough dimension in a range of from 6 microns to 30 microns.
Dependencies are inferred from the claim text.