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US 2024/0228947 A1Published application
Bacterial strains having fungicidal activity, compositions comprising same and use thereof
AssigneeLavie Bio Ltd
InventorsAda Viterbo Fainzilber, Amir Bercovitz, Galit Kuznets, Anna Movtchan, Hadar Kimelman, Michael Ionescu
Life of this patent
Estimating the term…
Abstract
The present invention relates to the field of biocontrol of plant pathogenic fungi, particularly to bacterial strains effective in treating and/or preventing plant diseases associated with phytopathogenic fungi and/or oomycetes; preparations, lysates and extracts thereof, compositions comprising same and use thereof.
Claims29
A bacterial preparation comprising a plurality of at least one isolated bacterial strain or a functional homolog thereof at a concentration which exceeds that found in nature, wherein the isolated bacterial strain is selected from the group consisting of:
- (1) strain LAV58567, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43954 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:5;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:36-38;
- and any combination thereof,
- (2) strain LAV104662, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43955 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:4;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:33-35;
- and any combination thereof,
- (3) strain LAV104858, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43956 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:3;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:30-32;
- and any combination thereof,
- (4) strain LAV104632, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43957 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:2;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in SEQ ID NO:29;
- and any combination thereof,
- (5) strain LAV104629, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43958 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:1;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:27-28;
- and any combination thereof,
- (6) strain LAV1000636, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43964 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:6;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in SEQ ID NO:39;
- and any combination thereof,
- (7) strain LAV1000506, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43965 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:7;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:40-42;
- and any combination thereof,
- (8) strain LAV1005101, the strain being selected from the group consisting of:
- a. a strain deposited under Accession Number 43959 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:13;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:62-66;
- and any combination thereof,
- (9) strain LAV1001107, the strain being selected from the group consisting of:
- a. a strain deposited under Accession Number 43960 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:12;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:57-61;
- and any combination thereof,
- (10) strain LAV1001081, the strain being selected from the group consisting of:
- a. a strain deposited under Accession Number 43961 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:11;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in SEQ ID NO:56;
- and any combination thereof,
- (11) strain LAV1000847, the strain being selected from the group consisting of:
- a. a strain deposited under Accession Number 43962 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:9;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:48-50;
- and any combination thereof,
- (12) strain LAV1000965, the strain being selected from the group consisting of:
- a. a strain deposited under Accession Number 43963 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:8;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:43-47;
- and any combination thereof,
- (13) strain LAV1000933, the strain being selected from the group consisting of:
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:10;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:51-55;
- and a combination thereof,
- (14) strain LAV104661, the strain being selected from the group consisting of
- d. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:14;
- e. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:67-68;
- and any combination thereof,
- (15) strain LAV104665, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:15;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:69-71;
- and a combination thereof,
- (16) strain LAV104794, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:16;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:72-74;
- and a combination thereof,
- (17) strain LAV104618, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:17;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:75-77;
- and a combination thereof,
- (18) strain LAV104692, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:18;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:78-80;
- and a combination thereof,
- (19) strain LAV104891, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:19;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:81-83;
- and a combination thereof,
- (20) strain LAV104630, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:20;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:84-85;
- and a combination thereof,
- (21) strain LAV104664, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:21;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:86-88;
- and a combination thereof,
- (22) strain LAV104922, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:22;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:89-91;
- and a combination thereof,
- (23) strain LAV59524, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:23;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:92-94;
- and a combination thereof,
- (24) strain LAV104961, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:25;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:95-97;
- and a combination thereof,
- (25) strain LAV104960, the strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:24;
- and
- (26) strain LAV104533, the strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:26.
108 more elements
5-9. (canceled)
(canceled)
(canceled)
17-18. (canceled)
20-21. (canceled)
A method for at least one of enhancing and/or conferring resistance to a plant or a part thereof toward a disease caused by a phytopathogenic fungus and/or oomycete, and preventing or treating a plant disease caused by the phytopathogenic fungus and/or oomycete, comprising contacting the plant, a part thereof or the habitat of said plant with at least one bacterial strain or a functional homolog thereof, a bacterial preparation comprising same, a lysate thereof, an extract thereof, a whole cell broth thereof, or an agricultural composition comprising same, wherein the bacterial strain is selected from the group consisting of:
- (1) strain LAV58567, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43954 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:5;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:36-38:
- and any combination thereof;
- (2) strain LAV104662, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43955 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:4;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:33-35:
- and any combination thereof;
- (3) strain LAV104858, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43956 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:3;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:30-32:
- and any combination thereof;
- (4) strain LAV104632, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43957 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:2;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in SEQ ID NO:29:
- and any combination thereof;
- (5) strain LAV104629, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43958 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:1;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:27-28:
- and any combination thereof;
- (6) strain LAV1000636, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43964 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:6;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in SEQ ID NO:39:
- and any combination thereof;
- (7) strain LAV1000506, the strain being selected from the group consisting of
- a. a strain deposited under Accession Number 43965 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:7;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:40-42:
- and any combination thereof;
- (8) strain LAV1005101, the strain being selected from the group consisting of:
- a. a strain deposited under Accession Number 43959 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:13;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:62-66:
- and any combination thereof;
- (9) strain LAV1001107, the strain being selected from the group consisting of:
- a. a strain deposited under Accession Number 43960 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:12;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:57-61:
- and any combination thereof;
- (10) strain LAV1001081, the strain being selected from the group consisting of:
- a. a strain deposited under Accession Number 43961 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:11;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in SEQ ID NO:56:
- and any combination thereof;
- (11) strain LAV1000847, the strain being selected from the group consisting of:
- a. a strain deposited under Accession Number 43962 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:9;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:48-50:
- and any combination thereof;
- (12) strain LAV1000965, the strain being selected from the group consisting of:
- a. a strain deposited under Accession Number 43963 at NCIMB;
- b. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:8;
- c. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:43-47;
- and any combination thereof;
- (13) strain LAV1000933, the strain being selected from the group consisting of:
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:10;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:51-55:
- and a combination thereof;
- (14) strain LAV104661, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:14;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:67-68:
- and any combination thereof;
- (15) strain LAV104665, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:15;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:69-71:
- and a combination thereof;
- (16) strain LAV104794, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:16;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:72-74:
- and a combination thereof;
- (17) strain LAV104618, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:17;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:75-77:
- and a combination thereof;
- (18) strain LAV104692, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:18;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:78-80:
- and a combination thereof;
- (19) strain LAV104891, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:19;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:81-83:
- and a combination thereof;
- (20) strain LAV104630, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:20;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:84-85:
- and a combination thereof;
- (21) strain LAV104664, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:21;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:86-88:
- and a combination thereof;
- (22) strain LAV104922, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:22;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:89-91:
- and a combination thereof;
- (23) strain LAV59524, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:23;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:92-94:
- and a combination thereof;
- (24) strain LAV104961, the strain being selected from the group consisting of
- a. a strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:25;
- b. a strain comprising at least one genomic marker comprising the nucleic acid sequence set forth in any one of SEQ ID NOs:95-97:
- and a combination thereof;
- (25) strain LAV104960, the strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:24;
- and
- (26) strain LAV104533, the strain comprising a 16S-rRNA sequence comprising the nucleic acid sequence set forth in SEQ ID NO:26.
108 more elements
23-31. (canceled)
The method of 22, wherein the phytopathogenic fungus is of a genus selected from the group consisting of Fusarium, Botrytis, Erysiphe, Aspergillus, and Rhizopus and wherein the oomycete is of a genus selected from the group consisting of Plasmopara, Pythium, Phytophthora, and Pseudoperonospora.
33-35. (canceled)
42-43. (canceled)
A method of obtaining a modified bacterial strain having an improved capability to confer or enhance the resistance of a plant towards pathogenic fungi and/or oomycetes, or to protect the plant from a disease caused by the pathogenic fungi and/or oomycetes, as compared to a parent unmodified bacterial strain, comprising:
- (a) culturing a parent bacterial strain having a capability to confer or enhance the resistance of a plant towards pathogenic fungi and/or oomycetes, or to protect the plant from a disease caused by the pathogenic fungi and/or oomycetes, under conditions suitable for expanding a population of the bacterial strain and allowing development of at least one bacterial mutant;
- and
- (b) selecting at least one bacterial mutant resultant of step (a) for an improved capability to confer or enhance the resistance of a plant towards the pathogenic fungi and/or oomycetes, or to protect the plant from a disease caused by said pathogenic fungi and/or oomycetes,
- thereby obtaining the modified bacterial strain having the improved capability to confer or enhance the resistance of a plant towards said pathogenic fungi and/or oomycetes, or to protect the plant from a disease caused by said pathogenic fungi and/or oomycetes as compared to the unmodified parent bacterial strain.
1 more element
45-46. (canceled)
Dependencies are inferred from the claim text.