Disposable cell removal system

A filtration system, comprising:

• a filtration unit comprising a housing with a sample inlet, a sample outlet, one or more valves, a first actuator, and a second actuator and a third actuator;
• and
• a cassette adapted to fit the filtration unit, the cassette comprising:
• a filter;
• a first reservoir in fluid communication with the filter, the first reservoir being arranged to couple to the first actuator for imparting flow to a cell-containing solution in a first direction through the filter;
• a second reservoir in fluid communication with the filter, the second reservoir being arranged to couple to the second actuator for imparting flow to a cell-containing solution in a second direction through said filter, wherein the second direction opposes the first direction;
• a third reservoir arranged to receive an amount of cell-free solution, the third reservoir being arranged to couple to the third actuator for imparting a suction force substantially simultaneously to the actuation of the first and/or the second actuators, wherein the third reservoir is in fluid communication with the sample outlet when connected to the filtration unit;
• wherein when the cassette is connected to the filtration unit, the filter is in fluid communication with the sample inlet and the sample outlet;
• and
• wherein the cassette is disposable and housed by a connector being removably adjointable to the filtration unit.