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US 2024/0226877 A9Published application
Diagnostics for Emerging Disease
AssigneeTrellis Bioscience, LLC
InventorsUrs A. Ramel, Joel Blatt, Lawrence M. Kauvar, Stote Ellsworth, Ralph A. Tripp, Leslie P. Jones
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Abstract
The present disclosure relates to enhancements in diagnostic assays for detection of analytes. The improved assays are suitable for management of infections and pandemics in humans and animal reservoirs, including but not limited to SARS-CoV-2 (CoV2), and for measurement of biomarkers of disease (both human and veterinary).
Claims67
A microfluidic device comprising:
- a housing;
- a substrate adapted to be disposed within the housing, the substrate comprising:
- a sample port adapted to receive a sample and extract at least one analyte from the sample into a liquid assay sample,
- a sample well coupled to the sample port,
- a reaction well, a first microfluidic channel coupled at a proximal end to the sample well, and a second microfluidic channel coupled at a distal end to the reaction well, wherein a distal end of the first microfluidic channel and a proximal end of the second microfluidic channel are isolated from each other via a fluid channel seal,
- a controller adapted to break the fluid channel seal and to meter the liquid assay sample from the sample well into the reaction well for an assay.
3 more elements
A method of performing an assay comprising:
- extracting an analyte from a sample received in a sample well via an extraction mixture into a liquid assay sample;
- connecting a first microfluidic channel to a second microfluidic channel via a controller, the first microfluidic channel coupled to the sample well at a proximal end and the second microfluidic channel coupled to a reaction well at a distal end, wherein the controller breaks a fluid channel seal disposed between a distal end of the first microfluidic channel and a proximal end of the second microfluidic channel;
- metering the liquid assay sample from the sample well into the reaction well via the controller;
- and
- assaying the liquid assay sample in the reaction well.
2 more elements
Dependencies are inferred from the claim text.