Therapeutic agent using genome-edited pluripotent stem cell
AssigneeKeio University
InventorsMasahiro TODA, Hideyuki OKANO, Ryota TAMURA, Mizuto SATO, Masahiro YO
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Abstract
To treat brain dysfunctions, brain diseases or tumors, the present invention provides a cell preparation comprising neural stem cells differentiated from pluripotent stem cells into which a suicide gene has been introduced.
Claims38
A cell preparation for treating central nervous system diseases/damages, which comprises neural stem cells differentiated from pluripotent stem cells into which a suicide gene has been introduced.
A method for preparing neural stem cells, comprising the following steps (1) and (2):
- (1) inserting a suicide gene immediately 3′ to a translation region of β-actin gene in pluripotent stem cells by genome editing;
- and
- (2) differentiating the pluripotent stem cells obtained in the step (1) into neural stem cells.
A method for selecting neural stem cells with high migratory property and/or anisotropic property for a tumor or damage site, wherein the expression level of at least one selected from ephrin A, ephrin A receptor, ephrin B receptor, ephrin B, and CXC motif chemokine receptor 4 is used as an indicator.
Dependencies are inferred from the claim text.