Transketolase epitopes, methods, and devices for igg-based tuberculosis serodiagnosis
AssigneeWayne State University
InventorLobelia Samavati
Life of this patent
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Abstract
The present disclosure relates generally to compositions, methods, devices, and systems for the diagnosis of tuberculosis in a subject. Also described is the development of direct ELISA systems for detecting IgG against specific sequences, such as SEQ ID NO: 1, SEQ ID NO: 12 (TKTμ; DLSSEVATHQPIIACLP), SEQ ID NO: 3 (TKT1; GEDGPTHQPIEHLSA), or SEQ ID NO: 5 (TKT3; HDSIGLGEDGPTHQPIEHLS).
Claims18
A method of detecting antibodies that specifically bind a Mycobacterium tuberculosis (M.tb) peptide in a sample of a subject suspected of having an M.tb infection, comprising detecting in the sample presence of an antibody that reacts with (specifically binds to) a peptide comprising (or having) the sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 12 (TKTμ;
- DLSSEVATHQPIIACLP), SEQ ID NO:
- 3 (TKT1;
- GEDGPTHQPIEHLSA), or SEQ ID NO:
- 5 (TKT3;
- HDSIGLGEDGPTHQPIEHLS).
A method for aiding in diagnosis of active Mycobacterium tuberculosis (M.tb) infection in a subject, comprising:
- contacting a sample from the subject with a peptide comprising the sequence of SEQ ID NO:
- 1, 12, 3, or 5 under conditions suitable for an anti-transketolase (TKT) antibody in the sample to form a complex with the peptide;
- detecting a level of formation of the complex using an enzyme-linked immunosorbent (ELISA) assay comprising a detecting antibody, wherein the detecting antibody is a horseradish peroxidase (HRP) conjugated anti-human IgG antibody, and a substrate to detect HRP, thereby determining the level of the anti-transketolase antibody in the sample;
- and
- comparing the level of antibody in the sample to a control level, wherein a difference in the level of antibody in the sample relative to the control level is indicative of an increased likelihood of the subject having active M.tb infection.
An immunoassay kit for selectively detecting anti-transketolase antibody in a biological sample, the kit comprising:
- as capture reagent, a peptide comprising (or alternatively, consisting of) the sequence of SEQ ID NO:
- 1, 12, 3 or 5, wherein the peptide forms a complex with an anti-transketolase antibody in the biological sample;
- and
- as detection reagent, a detectable anti-human IgG antibody, wherein the detectable anti-human IgG antibody binds to anti-transketolase antibody in the complex.
Dependencies are inferred from the claim text.