A system and method for spatial analysis of leaf images

A method for spatial analysis of leaf images, comprising:

• acquiring one or more images from one or more leaves of one or more plants;
• generating 3-dimensional (3D) images from the acquired one or more images from associated spectral heatmaps;
• identifying a stem and a plurality of veins coupled to the stem in each generated 3D image;
• identifying i) one or more of valleys and ridges as regions between two consecutive veins from the plurality of veins, or ii) one or more of peaks and valleys as regions between two consecutive veins from the plurality of veins in the one or more generated 3D images;
• calculating average slopes in the generated 3D images between i) one or more of peak-to-valley, or ii) ridge-to-valley for each of the plurality of veins in each of the generated 3D images;
• calculating a nitrogen index based on the calculated average slopes;
• if the calculated nitrogen index is between two thresholds, communicate to a user to add a predetermined chemical to the one or more plants.