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US 2024/0210291 A1Published application
Microscope and observation method
AssigneeOsaka University
InventorsKatsumasa FUJITA, Yasuaki KUMAMOTO
Life of this patent
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Abstract
A microscope (1) according to the present embodiment includes: an objective lens (11) configured to receive light from a sample (200); and sample freezing means configured to freeze the sample (200) by supplying cryogen (160) to or bringing the cryogen (160) into contact with the sample (200) in the field of view of the objective lens (11). Use of the microscope (1) having such a configuration allows for freezing and observing a sample in the field of view of the objective lens (11).
Claims18
A microscope comprising:
- an objective lens configured to receive light from a sample;
- a sample holder holding the sample;
- and
- sample freezing means configured to freeze the sample by supplying cryogen to or bringing cryogen into contact with the sample in a field of view of the objective lens,
- wherein the sample holder includes a first plate including an opening for install the sample,
- the sample freezing means is disposed above the first plate,
- the sample freezing means includes a cryogen storage tub for storing the cryogen disposed directly above the opening, and
- the cryogen is supplied to the sample from above the sample by flowing the cryogen through a cryogen inlet disposed above the cryogen storage portion.
5 more elements
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An observation method comprising:
- a step of arranging a sample held by a sample holder in a field of view of an objective lens;
- a step of freezing the sample by supplying or moving cryogen to the sample in a field of view of the objective lens;
- and
- a step of observing the frozen sample wherein the sample holder includes a first plate including an opening for install the sample,
- the sample freezing means is disposed above the first plate,
- the sample freezing means includes a cryogen storage tub for storing the cryogen disposed directly above the opening, and
- the cryogen is supplied to the sample from above the sample by flowing the cryogen through a cryogen inlet disposed above the cryogen storage portion.
4 more elements
A microscope comprising:
- an objective lens configured to receive light from a sample;
- and
- sample freezing means configured to freeze the sample by supplying cryogen to or bringing cryogen into contact with the sample in a field of view of the objective lens,
- wherein the sample is irradiated with light and thereby at least a portion of the frozen sample is melted.
1 more element
Dependencies are inferred from the claim text.