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US 2024/0209435 A1Published application
Methods of identifying combinations of transcription factors
AssigneePresident and Fellows of Harvard College
InventorsHon Man Alex Ng, George M. Church, Parastoo Khoshakhlagh
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Abstract
Provided herein, in some embodiments, are methods and compositions for identifying combinations of transcription factors, for example, those involved in cell type conversion processes, such as cell differentiation.
Claims21
-75. (canceled)
A method for inducing a change in a transcriptomic state of a population of pluripotent stem cells, the method comprising:
- a. contacting the population of pluripotent stem cells with one or more enzymes and one or more vectors, wherein the one or more vectors comprise:
- (i) one or more nucleic acids encoding one or more transcription factors, wherein at least a subset of the one or more transcription factors induces the change in the transcriptomic state of the population of pluripotent stem cells;
- b. contacting the one or more nucleic acids encoding the one or more transcription factors with the one or more enzymes;
- and
- c. determining the change in the transcriptomic state of the population of pluripotent stem cells compared to a control cell, wherein the change in the transcriptomic state of the population of pluripotent stem cells is detected in at least 14% of the population of pluripotent stem cells, thereby differentiating the population of pluripotent stem cells.
2 more elements
A population of pluripotent stem cells, wherein the population of pluripotent stem cells comprise one or more enzymes and one or more vectors, wherein the one or more vectors comprise:
- a. one or more nucleic acids encoding one or more transcription factors, wherein at least a subset of the one or more transcription factors induce a change in a transcriptomic state of the population of pluripotent stem cells;
- wherein contacting the population of pluripotent stem cells with the one or more vectors and the one or more enzymes induce the change in the transcriptomic state, and wherein the change is detected in at least 14% of the population of pluripotent stem cells compared to a control cell, thereby differentiating the population of pluripotent stem cells.
Dependencies are inferred from the claim text.