Enzymatic synthesis of ntp and nqp
AssigneeCodexis, Inc.
InventorsDavid Entwistle, Stephanie Marie Forget, Anders Matthew Knight, Mikayla Jianghongxia Krawczyk, Marissa Greene MacAvoy, Simon Ng, Nicholas Porter, Philip Provencher, Amani Shoubber, Leann Quertinmont Teadt, Jonathan Vroom, David Watts, Leland Ken Wong
Life of this patent
Estimating the term…
Abstract
The present disclosure provides enzymatic methods for the production of 3′-phosphate-nucleoside-5′-triphosphate (NQP). The present disclosure further provides enzymatic methods for the production of nucleotide-5′-triphosphates.
Claims58
A method of producing a nucleotide triphosphate with a phosphate group at the 3′ position of the sugar (NQP), the method comprising at least:
- contacting a nucleoside diphosphate (NDP) with a nucleoside diphosphate kinase, a 3′-O-kinase, and a phosphate donor under reaction conditions such that a NTP with a phosphate group on the 3′ position of the sugar moiety (NQP) is produced.
A method of producing a NTP with a phosphate group at the 3′ position of the sugar (NQP), the method comprising contacting a nucleoside with a nucleoside kinase, a nucleoside monophosphate kinase, nucleoside diphosphate kinase, and a 3′-O-kinase in presence of a phosphate donor under reaction conditions such that a product NTP with a phosphate group at the 3′ position of the sugar (NQP) is produced.
A method of producing a NTP with a phosphate group at the 3′ position of the sugar (NQP), the method comprising (i) contacting a nucleoside with a nucleoside kinase, a nucleotide monophosphate kinase, and a nucleoside diphosphate kinase in presence of a phosphate donor under reaction conditions such that a NTP is produced;
- and iii) contacting the NTP produced in (i) with a 3′-O-kinase enzyme under suitable reaction conditions, such that a NTP with a phosphate group at the 3′ position of the sugar (NQP) is produced.
A method of producing a NTP with a phosphate group at the 3′ position of the sugar (NQP), the method comprising (i) contacting a nucleoside with a nucleoside kinase, (ii) contacting the product of (i) with a nucleotide monophosphate kinase, (iii) contacting the product of (ii) with a nucleoside diphosphate kinase, and (iv) contacting the product of (iii) with a 3′-O-kinase enzyme, wherein (i), (ii), (iii) and (iv) are carried out in presence of a phosphate donor under reaction conditions such that a product NTP with a phosphate group at the 3′ position of the sugar (NQP) is produced.
47-70. (canceled)
A method of producing a nucleoside triphosphate (NTP), comprising contacting a nucleoside with a nucleoside kinase, a nucleoside monophosphate kinase, and a nucleoside diphosphate kinase in presence of a phosphate donor under reaction conditions such that NTP is produced.
72-86. (canceled)
Dependencies are inferred from the claim text.