Mammalian cell line and method for producing soluble e2 recombinant antigen of classical swine fever virus using the same and application thereof
AssigneeNational Pingtung University of Science and Technology
InventorsHso-Chi Chaung, Wen-Bin Chung, Chi-Chih Chen
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Abstract
The present disclosure relates to a mammalian cell line and a method for producing soluble E2 recombinant antigen of classical swine fever virus using the same. A mammalian cell expression system with different cell passage numbers can stably express a large amount of soluble CSFV-E2 recombinant protein, effectively reducing the production cost of CSFV-E2 recombinant protein, and then applied to the production of CSFV-E2 protein subunit vaccines.
Claims12
A mammalian cell line stably expressing soluble E2 recombinant antigen of classical swine fever virus, which is Chinese hamster ovary cell line CCC_E2_5E12 deposited in German Collection of Microorganisms and Cell Cultures GmbH (Deutsche Sammlung von Mikroorganismen und Zellkulturen, DSMZ) (Inhoffenstraße 7B, 38124 Braunschweig, Science Campus Braunschweig-Süd, GERMANY) on May 17, 2023 and assigned accession number DSM ACC3376.
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