Universal linker reagents for dna synthesis

A method for synthesis of oligonucleotides comprising:

• (a) providing an electrode device with a planar surface,
• (b) depositing carboxylic acid electrochemically reducing the carboxylic acid onto the planar surface,
• (c) depositing an amine coating onto the deposited carboxylic acid, wherein the planar surface comprises silicon titanium. or platinum;
• (d) coupling the surface with a universal linker;
• and
• (e) synthesizing the oligonucleotide.

A method for synthesis of oligonucleotide primer pairs comprising

• providing a solid support comprising a first universal linker immobilized on a surface of the solid support,
• performing a first phosphoramidite DNA synthesis to generate a first oligonucleotide primer, wherein the 3′ end of the first oligonucleotide primer is attached to the first universal linker,
• coupling a second universal linker to the 5′ end of the first oligonucleotide primer,
• performing a second phosphoramidite DNA synthesis to generate a second oligonucleotide primer, wherein the 3′ end of the second oligonucleotide primer is attached to the second universal linker, and
• contacting the solid support with a releasing agent thereby releasing the first and the second oligonucleotide primers from the solid support, wherein each of the released first oligonucleotide primer and the released second oligonucleotide primer comprises a 3′-hydroxy group,
• wherein the first universal linker is immobilized to the solid support by reacting the solid support with a first compound of
• wherein
• A is
• X is acyl, aroyl, or silyl;
• and
• Y is dimethoxytrityl group or a protecting group removable under acidic or neutral conditions:
• wherein
• A is
• X is acyl, aroyl, or silyl;
• and
• Y is dimethoxytrityl group or a protecting group removable under acidic or neutral conditions;
• or combinations thereof.

A compound of Formula (XI),

• wherein
• A is
• X is acyl, aroyl, or silyl, and
• Y is dimethoxytrityl group or a protecting group removable under acidic or neutral conditions.