Cartilage treatment for use in eyelid reconstruction
AssigneeThe Regents of the University of California
InventorJustin KARLIN
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Abstract
The present disclosure relates to methods for preparing car cartilage for eyelid reconstruction, including upper eyelid reconstruction, after trauma, disease, or tumor excision. The present disclosure also relates to compositions, uses, and methods for reconstructing an eyelid. The present disclosure also relates to kits for preparing ear cartilage for eyelid reconstruction. The disclosed compositions, uses, kits, and methods may also be used for autograft, allograft, isograft, and xenograft transplantation, for stored or banked grafts, and for other therapeutic uses.
Claims63
A method for preparing ear cartilage for eyelid reconstruction comprising the steps of:
- (a) harvesting ear cartilage from the scaphoid fossa or the conchal bowl and removing the perichondrium;
- (b) preparing a strip of cartilage of about 4-8 mm×about 10-20 mm and about 0.5 mm thick for the upper eyelid, or about 3-6 mm×about 10-20 mm and about 0.5 mm thick for the lower eyelid;
- (c) incubating the cartilage strip in a buffered elastase solution;
- and
- (d) washing the cartilage strip.
Th method of clam 10 wherein the incubating is from about 30 minutes to about 24 hours.
A composition for reconstructing an eyelid, the composition comprising scaphoid fossa ear cartilage or conchal bowl ear cartilage cut into a strip of about 4-8 mm×about 10-20 mm and about 0.5 mm thick for the upper eyelid, or about 3-6 mm×about 10-20 mm and about 0.5 mm thick for the lower eyelid, following removal of residual perichondrium, incubated in a buffered elastase solution, and washed, wherein the instantaneous Young modulus of the strip following washing is between about 100-1000 MPa, such as between about 100 and 500 MPa, and the equilibrium Young modulus of the strip following washing is between about 1 and 10 MPa, such as between about 1 and 5 MPa.
A kit for reconstructing an eyelid from an ear cartilage, the kit comprising:
- (a) a buffered elastase solution;
- (b) a buffered elastase inhibitor solution;
- and
- (c) optionally, a buffered wash solution.
A method for preparing ear cartilage for eyelid reconstruction comprising the steps of:
- (a) harvesting ear cartilage from the scaphoid fossa or the conchal bowl and removing the perichondrium;
- (b) incubating the cartilage in a buffered elastase solution and optionally thereafter washing the cartilage;
- and
- (c) preparing a strip of cartilage of about 4-8 mm×about 10-20 mm and about 0.5 mm thick for the upper eyelid, or about 3-6 mm×about 10-20 mm and about 0.5 mm thick for the lower eyelid.
Th method of clam 42 wherein the incubating is from about 30 minutes to about 24 hours.
A composition for reconstructing an eyelid, the composition comprising scaphoid fossa ear cartilage or conchal bowl ear cartilage incubated in a buffered elastase solution, washed, and cut into a strip of about 4-8 mm×about 10-20 mm and about 0.5 mm thick for the upper eyelid, or about 3-6 mm×about 10-20 mm and about 0.5 mm thick for the lower eyelid, following removal of residual perichondrium before or after elastase treatment, wherein the instantaneous Young modulus of the strip following washing is between about 100-1000 MPa, such as between about 100 and 500 MPa, and the equilibrium Young modulus of the strip following washing is between about 1 and 10 MPa, such as between about 1 and 5 MPa.
Dependencies are inferred from the claim text.