Personalized immunotherapy using intestinal metabolites
OwnerBethany HENRICK, Petter BRODINinventors
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Abstract
Disclosed is a T cell polarizing composition comprising a plurality of metabolites selected from metabolites enriched in non-dysbiotic feces, preferably including one or more of acetate, lactate, indole 3 lactate, 3-(4-hydroxyphenyl)lactate, or phenyllactate, bile acids (cholate, chenodeoxycholate, cholate sulfate), or IFNbeta. Further disclosed is a method of producing the T cell polarizing composition comprising mixing interferon beta (IFNbeta), acetate, lactate, and/or ILA.
Claims65
A T cell polarizing composition comprising a plurality of metabolites selected from metabolites enriched in non-dysbiotic feces, preferably including one or more of acetate, lactate, indole 3 lactate, 3-(4-hydroxyphenyl)lactate, or phenyllactate, bile acids (cholate, chenodeoxycholate, cholate sulfate), or IFNβ.
A T cell preparation comprising:
- a. cultured human T regulatory and helper T cells polarized from a purified naïve T cell population;
- and
- b. an activity profile comprising desired frequency or ratio of T regulatory and T helper cell types.
A method of making a T cell preparation, wherein the composition is made ex vivo, said method of making T cell preparation comprising
- a. collecting blood;
- b. isolating naïve T cells or enriching naïve T cells;
- c. exposing naïve T cells to a polarizing composition;
- d. recovering polarized T cells;
- and
- e. optionally administering a composition of the T cell preparation to a subject in need thereof.
A method of enhancing immune expansion and/or reducing intestinal inflammation in a human subject in need, comprising administering the T cell polarizing composition or T cell preparation of any one of the preceding claims to said subject.
A method of any one of claims 43-55, wherein the patient is has a chronic viral infection.
A method of 60, wherein the polarizing composition further comprises microbiome modulators delivered to the gut, wherein microbiome modulators are selected from a prebiotic, probiotic and/or post-biotic preparations.
A method of increasing and/or maintaining a level of circulating T cell population in a mammal, comprising:
- a. selecting a mammal having insufficient Regulatory T cells;
- b. collecting and isolating naïve T cell;
- c. applying a composition to polarize naïve T cells to Th1 and T regulatory cells;
- and
- d. administering to the mammal the T cell preparation.
Dependencies are inferred from the claim text.