# US 2024/0201198 A1 · Compositions, methods, and utility of conjugated biomolecule barcodes > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2024/0201198 A1 - Document ID: US20240201198A1 - Status: Published application - Kind: A1 - Application number: 18/518,854 - Filed: 2023-11-24 - Published: 2024-06-20 - Earliest claimed: 2021-05-26 - Assignees: Board of Regents, The University of Texas System; Erisyon, Inc. - Applicants: Board of Regents, the University of Texas System; Erisyon, Inc. - Inventors: Edward MARCOTTE; Eric V. ANSLYN; Jagannath SWAMINATHAN; Cecil J. HOWARD, II; Angela M. BARDO; Zachary Booth SIMPSON - Web page: https://patentgrep.com/patent/US20240201198A1 ## CPC - G01N 33/6803: {General methods of protein analysis not limited to specific proteins or families of proteins} - G01N 33/6824: {involving N-terminal degradation, e.g. Edman degradation} - G01N 33/58: involving labelled substances (G01N33/53 takes precedence) - G01N 33/582: {with fluorescent label} - G01N 33/6821: {involving C-terminal degradation} - G01N 33/6848: {Methods of protein analysis involving mass spectrometry} - G01N 2333/9015: Ligases (6) - G01N 2458/10: Oligonucleotides as tagging agents for labelling antibodies - G01N 2458/15: Non-radioactive isotope labels, e.g. for detection by mass spectrometry - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract The present disclosure provides a range of methods for dense information storage in oligomeric constructs, such as peptides. Various methods of the present disclosure provide for information storage in oligomers and oligomer libraries, and information retrieval by subsequent oligomer sequencing or analysis. The present disclosure further provides methods for appending information to materials and molecules with analyzable oligomeric constructs. ## Claims ### Claim 1 A method for identifying a biomolecule, said method comprising: (a) providing said biomolecule having coupled thereto an oligopeptide barcode associated with the biomolecule's identity, wherein said oligopeptide barcode comprises a plurality of amino acid residues, wherein at least a subset of said amino acid residues comprise a label; and (b) identifying said label(s) by sequencing by degradation, thereby identifying the oligopeptide and the biomolecule coupled thereto. ### Claim 2 (depends on 1) The method of claim 1, wherein said biomolecule is a polypeptide. ### Claim at position 3 3-7. (canceled) ### Claim 8 (depends on 1) The method of claim 1, wherein said biomolecule is coupled to said oligopeptide barcode by co-expression or co-translation of said oligopeptide barcode as a peptide tag. ### Claim 9 (canceled) ### Claim 10 (depends on 1) The method of claim 1, wherein said biomolecule is coupled to said oligopeptide by chemical synthesis. ### Claim at position 7 11-12. (canceled) ### Claim 13 (depends on 1) The method of claim 1, wherein said oligopeptide barcode comprises from about 2 to about 30 amino acids. ### Claim 14 (depends on 1) The method of claim 1, wherein said oligopeptide barcode comprises a non-natural amino acid. ### Claim 15 (canceled) ### Claim 16 (depends on 1) The method of claim 1, wherein said label is coupled to an internal amino acid residue of said plurality of amino acid residues. ### Claim 17 (depends on 1) The method of claim 1, wherein said label is an amino acid specific label. ### Claim 18 (depends on 17) The method of claim 17, wherein said amino acid specific label comprises a methionine specific label, an arginine specific label, a histidine specific label, a tyrosine specific label, a carboxylic acid R-group specific label, a lysine specific label, a cysteine specific label, a tryptophan specific label, or any combination thereof. ### Claim 19 (depends on 17) The method of claim 17, wherein said amino acid specific label comprises a non-natural amino acid specific label. ### Claim 20 (depends on 19) The method of claim 19, wherein said non-natural amino acid specific label is a phosphoserine specific label, phosphothreonine specific label, pyroglutamic acid specific label, hydroxyproline specific label, azidolysine specific label, or dehydroalanine specific label. ### Claim at position 16 21-25. (canceled) ### Claim 26 (depends on 1) The method of claim 1, wherein said label generates at least one signal or at least one signal change. ### Claim 27 (depends on 26) The method of claim 26, wherein said at least one signal or said at least one signal change is an optical signal. ### Claim 28 (depends on 26) The method of claim 26, wherein said at least one signal or said at least one signal change comprises a plurality of signals of different intensities. ### Claim 29 (canceled) ### Claim 30 (depends on 1) The method of claim 1, wherein said sequencing by degradation comprises enzymatic cleavage of said oligopeptide barcode from said biomolecule. ### Claim 31 (depends on 1) The method of claim 1, wherein said sequencing by degradation comprises chemical cleavage of said oligopeptide barcode from said biomolecule. ### Claim 32 (canceled) ### Claim 33 (depends on 1) The method of claim 1, wherein said oligopeptide barcode is coupled to said biomolecule via an N-terminal tag, a C-terminal tag, or an amino acid sidechain. ### Claim at position 25 34-50. (canceled) ### Claim 51 A method comprising: (a) providing a polypeptide immobilized to a support, wherein said polypeptide comprises at least one labeled internal amino acid, and wherein said polypeptide encodes data; (b) detecting at least one signal or signal change from said polypeptide immobilized to said support to identify at least a portion of a sequence of said polypeptide; or (c) subjecting said polypeptide to conditions sufficient to remove at least one amino acid from said polypeptide. ### Claim at position 27 52-91. (canceled)