Detection assay
OwnerPeter Fitzgerald, John Victor Lamont +4inventors
InventorsPeter Fitzgerald, John Victor Lamont, Ivan McConnell, Ciaran Richardson, Philip Lowry, Jim Curry
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Abstract
The present invention relates to improved detection assays via the utilisation of a substrate with an analyte immobilised on its surface by means of a linking agent and a cross-linker. The present invention also relates to a method of producing a substrate for use in detection assays and a method of detecting an analyte using said substrate.
Claims18
A substrate comprising a plurality of discrete reaction zones onto which one or more biological molecules are immobilised to the substrate surface by means of a linking agent and a cross-linker, wherein the cross-linker is a sulphone-based cross-linker having a structure according to Formula (1):
- wherein X is —CH2—;
- Y is methylidene (═CH2), or Y is selected from —CH2—SO2-Ph-R4, —CH2—SO2-Ph-CH3, —CH2—SO2—CH3, —CH2—O—SO2-Ph-R4, —CH2—O—SO2-Ph-CH3 or —CH2—O—SO2—CH3;
- R1 is CH3 or CH3-Ph, wherein the Ph is optionally substituted with one or more C1-6 alkyl, NO2, F, Cl or Br;
- and
- R2 is selected from —C6-12aryl-Z, —C1-18alkyl-Z, and —C2-20alkenyl-Z, wherein Z is selected from COR3, —NH2 and —OH, R3 is selected from H, OH, NH2, —C1-6alkyl-OH and (EtO)3Si—(CH2)n—NH— in which n is 1-6;
- and R4 is H or is optionally substituted with one or more C1-6alkyl, NO2, F, Cl or Br.
A method for producing a substrate having a biological molecule immobilised thereon, comprising attaching the biological molecule to the substrate surface by means of a sulphone-based cross-linker having a structure according to Formula (1).
A method of detecting the presence of an analyte in a sample obtained from a subject, wherein said analyte has affinity for a biological molecule, or portion thereof, and wherein said method comprises bringing the sample obtained from the subject into contact with the biological molecule, or portion thereof, which is immobilised on a substrate support, detecting the binding of the analyte to the biological molecule, or portion thereof, via means of a detectably-labelled molecule, and measuring the amount of binding of said analyte compared to a control, wherein the biological molecule, or portion thereof, is immobilised to the substrate surface by means of a linking agent and a cross-linker, wherein the cross-linker is a sulphone-based cross-linker having a structure according to Formula (1).
Dependencies are inferred from the claim text.