# US 2024/0201147 A1 · Method for detecting tcep content in adc by lc-ms/ms

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- Number: US 2024/0201147 A1
- Document ID: US20240201147A1
- Status: Published application
- Kind: A1
- Application number: 17/794,629
- Filed: 2022-03-30
- Published: 2024-06-20
- Earliest claimed: 2021-03-31
- Assignees: Remegen Co., Ltd.
- Applicants: Remegen Co., Ltd.
- Inventors: Xuejing YAO; Li WANG; Wenlong SUN; Guiping QI
- Web page: https://patentgrep.com/patent/US20240201147A1

## CPC

- G01N 30/7233: {interfaced to liquid or supercritical fluid chromatograph (interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04)}
- G01N 30/88: Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N 2030/027: {Liquid chromatography}
- G01N 2030/884: {organic compounds}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

The present disclosure relates to a method for detecting TCEP content in ADC by LC-MS/MS. The ADC samples are derivatized by Ellman reaction, and then the TCEP content is measured by LC-MS/MS. This method can exclude the interference caused by the substances that may react with Ellman in complex ADC samples, which provides with no need for sample pretreatment, and with strong specificity.

## Claims

### Claim 1

A method for detecting TCEP content in ADC samples by liquid chromatography-tandem mass spectrometry (LC-MS/MS), wherein the detected ADC samples are derivatized by Ellman reaction, and a C18 chromatographic column is used, without need for sample pretreatment.

### Claim 2 (depends on 1)

The method according to claim 1, wherein a mobile phase A used in the C18 chromatographic column is an aqueous solution comprising formic acid; preferably an aqueous solution comprising 0.1% formic acid.

### Claim 3 (depends on 2)

The method according to claim 2, wherein a mobile phase B used in the C18 chromatographic column is an acetonitrile/methanol solution comprising formic acid; preferably an acetonitrile/methanol solution (80/20, v/v) comprising 0.1% formic acid.

### Claim 4 (depends on 3)

The method according to claim 3, wherein concentration of the added Ellman reagent is about 5-20 μg/ml, more preferably 10.0 μg/ml.

### Claim 5 (depends on 4)

The method according to claim 4, wherein the method uses TCEP to prepare standard solution.

### Claim 6 (depends on 5)

The method according to claim 5, wherein the standard solution is preferably a TCEP solution of 1.0 μg/ml.

### Claim 7 (depends on 6)

The method according to claim 6, wherein the preparation of the TCEP solution and the preparation of the series solutions for standard curve comprise the following steps:
1) Preparation of TCEP working solution: weighing 5˜10 mg of TCEP·HCl reference substance into an EP tube and dissolving with an appropriate amount of sample dissolving solution, and the weighed mass is expressed as m, calculating the TCEP concentration by a molar ratio of TCEP·HCl and TCEP of 1:1, and performing dilution stepwise to a TCEP concentration of 1.0 μg/ml;
2) Preparation of series solutions for standard curve: diluting 1.0 μg/ml TCEP standard solution with diluent to the TCEP standard solutions with concentrations of 16 ng/ml, 8 ng/ml, 4 ng/ml, 2 ng/ml, 1 ng/ml, and 0.5 ng/ml, respectively.

### Claim 8 (depends on 7)

The method according to claim 7, wherein the method further comprises using quality control solutions, which are prepared by diluting a 1.0 μg/ml TCEP solution with diluent, and the concentrations of the quality control solutions are 12 ng/ml, 3 ng/ml, and 1 ng/ml of TCEP quality control solutions.

### Claim 9 (depends on 7)

The method according to claim 7, wherein the diluent is a 50% aqueous methanol solution.

### Claim 10 (depends on 1)

The method according to claim 1, wherein the liquid chromatograph includes but is not limited to Agilent 1200 liquid chromatograph from Agilent USA, Teledyne Isco high pressure preparative liquid chromatograph, and Agilent HPLC 1260 high performance liquid chromatograph.

### Claim 11 (depends on 1)

The method according to claim 1, wherein the tandem mass spectrometer includes but is not limited to API 4000 tandem mass spectrometer from AB Sciex USA, Shimadzu triple quadrupole liquid chromatography mass spectrometer, and GBC inductively coupled plasma orthogonal acceleration time-of-flight mass spectrometer Optimass.

### Claim 12 (depends on 1)

The method according to claim 1, wherein the C18 chromatographic column includes but is not limited to YMC-C18, Agilent Extard-C18, and Shiseido PAK CR-18.