# US 2024/0201068 A1 · System for dissociation of solid biopsy and a method for inspection

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- Number: US 2024/0201068 A1
- Document ID: US20240201068A1
- Status: Published application
- Kind: A1
- Application number: 18/580,695
- Filed: 2022-06-23
- Published: 2024-06-20
- Earliest claimed: 2021-07-20
- Assignees: Max-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
- Applicants: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
- Inventors: Markéta ICHA KUBÁNKOVÁ; Despina SOTERIOU; Jochen Reinhold GUCK; Shada HOFEMEIER ABU HATTUM; Felix DIRLA
- Web page: https://patentgrep.com/patent/US20240201068A1

## CPC

- B01L 3/5027: {by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip}
- G01N 15/1459: {the analysis being performed on a sample stream}
- G01N 1/286: {involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising (microtomes G01N1/06; pulverising in general B02C; mixing in general B01F)}
- G01N 1/4077: {by other techniques involving separation of suspended solids}
- G01N 15/1433: using image recognition
- G01N 15/1484: {microstructural devices}
- G01N 33/5008: {for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics}
- B01L 2300/0627: Sensor or part of a sensor is integrated
- B01L 2300/0681: Filter
- B01L 2300/0877: Flow chambers
- B01L 2400/0409: centrifugal forces
- C12M 23/16: {Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093)}
- G01N 2001/4088: {filtration}
- G01N 2015/1006: {for cytology}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

The present invention relates to System for analysing tissue, comprising: a dissociation means for at least partially dissociating a tissue sample so as to obtain individual cells, a microfluidic flow device through which a fluid comprising the dissociated cells can be made to flow, and an evaluation device arranged to evaluate the cells as they are being transported through the microfluidic flow device, the system preferably being arranged for a label free analysis of tissue.

## Claims

### Claim 1

A system for analyzing tissue, comprising:
a dissociation means for at least partially dissociating a tissue sample so as to obtain individual cells;
a microfluidic flow device through which a fluid comprising the dissociated cells can be made to flow; and
an evaluation device arranged to evaluate the cells as they are being transported through the microfluidic flow device, the evaluation device comprising an imaging means arranged to obtain images of the cells as they are being transported through the microfluidic flow device.

### Claim 2 (depends on 1)

The system according to claim 1, the imaging means further comprising an image analysis device arranged for analyzing the images obtained of the cells, wherein the image analysis device is arranged to analyze the images in real time and/or wherein the image analysis device uses a supervised or unsupervised learning technique, for analyzing the images, the image analysis device being arranged for analyzing light scattering.

### Claim 3 (depends on 1)

The system according to claim 1, the evaluation device comprising an electrical properties measurement means arranged for measuring electrical properties of the cells as they are being transported through the microfluidic flow device, the electrical properties measurement means comprising an impedance measurement means arranged for measuring the impedance of the cells as they are being transported through the microfluidic flow device.

### Claim 4 (depends on 1)

The system according to claim 1, the system further comprising a filtering means arranged for filtering out debris created during the dissociation of the tissue sample.

### Claim 5 (depends on 1)

The system according to claim 1, the system being arranged so that the individual cells are made to flow through the microfluidic flow device as they are output by the dissociation means.

### Claim 6 (depends on 1)

The system according to claim 1, further comprising a means for adding a chemical substance to the dissociated cells prior to the cells being evaluated by the evaluation device.

### Claim 7 (depends on 1)

The system according to claim 1, the dissociation means using an enzymatic and/or mechanical dissociation method.

### Claim 8

A method of analyzing tissue, comprising:
dissociating tissue to be analyzed so as to obtain individual cells;
causing the dissociated cells to flow through a microfluidic channel; and
evaluating the cells as they flow through the microfluidic channel, wherein the evaluation of the cells includes the obtaining of images of the cells as they flow through the microfluidic channel.

### Claim 9 (depends on 8)

The method according to claim 8, wherein the images are analyzed in real time and/or wherein the images are analyzed using a neuronal network.

### Claim 10 (depends on 8)

The method according to claim 8, wherein the evaluation comprises measuring electrical properties of the cells as they flow through the microfluidic channel, wherein the impedance of the cells is measured as the cells flow through the microfluidic channel.

### Claim 11 (depends on 8)

The method according to claim 8, further comprising filtering the dissociated cells before they are caused to flow through the microfluidic channel.

### Claim 12 (depends on 8)

The method according to claim 8, wherein the dissociated cells are made to flow through the microfluidic channel as they are being dissociated.

### Claim 13 (depends on 8)

The method according to claim 8, further comprising a step of adding a chemical substance to the dissociated cells prior to the cells being evaluated.

### Claim 14 (depends on 8)

The method according to claim 8, wherein the cells are dissociated using an enzymatic and/or mechanical dissociation method.

### Claim 15 (depends on 1)

A method comprising screening chemical substances using the system of claim 1.

### Claim 16 (depends on 15)

The method according to claim 15, wherein the chemical substances comprise medicines.

### Claim 17 (depends on 8)

The method according to claim 8, further comprising screening chemical substances.

### Claim 18 (depends on 1)

The system according to claim 1 being arranged for a label free analysis of tissue.

### Claim 19 (depends on 1, 8)

The method according to claim 8 carried out using the device according to claim 1.

### Claim 20 (depends on 2)

The system according to claim 2, wherein the supervised or unsupervised learning technique is a neuronal network.

### Claim 21 (depends on 8)

The system according to claim 8, wherein the method of analyzing tissue carries out a label free analysis of tissue.