# US 2024/0201066 A1 · Particle analysis system and particle analysis method

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- Number: US 2024/0201066 A1
- Document ID: US20240201066A1
- Status: Published application
- Kind: A1
- Application number: 17/905,545
- Filed: 2021-02-16
- Published: 2024-06-20
- Earliest claimed: 2020-03-13
- Assignees: Sony Group Corporation
- Applicants: Sony Group Corporation
- Inventors: TOMOYUKI UMETSU; NAOKI IDE
- Web page: https://patentgrep.com/patent/US20240201066A1

## CPC

- G01N 15/1434: Optical arrangements
- G01N 15/1459: {the analysis being performed on a sample stream}
- G01N 15/01: specially adapted for biological cells, e.g. blood cells (investigating sedimentation of particle suspensions in blood G01N15/05)
- G01N 15/1429: Signal processing
- G01N 15/149: specially adapted for sorting particles, e.g. by their size or optical properties
- G01N 2015/1006: {for cytology}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A measurement spectrum obtained by irradiating a particle labeled with a plurality of fluorescent dyes with excitation light is separated for each fluorescent dye with high accuracy. Provided is a particle analysis system (1) including a plurality of photodetectors (23) configured to acquire light generated by irradiating a particle labeled with a plurality of fluorescent dyes with excitation light; and an information processing unit (132) configured to calculate fluorescence intensity of each fluorescent dye by performing separation processing on a measurement spectrum based on measured values from the plurality of photodetectors (23) with a single staining spectrum of each fluorescent dye, in which the separation processing is performed by using a weighted least squares method (WLSM) including a weight determined based on a variation in the measured values.

## Claims

### Claim 1

A particle analysis system including:
a plurality of photodetectors configured to acquire light generated by irradiating a particle labeled with a plurality of fluorescent dyes with excitation light; and
an information processing unit configured to calculate fluorescence intensity of each fluorescent dye by performing separation processing on a measurement spectrum based on measured values from the plurality of photodetectors with a single staining spectrum of each fluorescent dye,
wherein the separation processing is performed by using a weighted least squares method (WLSM) including a weight determined based on a variation in the measured values.

### Claim 2 (depends on 1)

The particle analysis system according to claim 1,
wherein the information processing unit calculates the fluorescence intensity by using an evaluation function represented by the following Formula (1)
[
x
1
⋮
x
n
]
=
(
[
S
T
]
[
L
]
[
S
]
)
-
1
[
S
T
]
[
L
]
[
y
1
⋮
y
m
]
(
1
)
in the formula, S represents a determinant indicating a shape of the single staining spectrum, ST represents a transposed determinant of S, L represents a determinant indicating the weight of the single staining spectrum, xn (n=1 to the number of fluorescent dyes) represents a fluorescence intensity of an n-th fluorescent dye, ym (m=1 to the number of photodetectors) represents a measured value of an m-th photodetector, max (yi, 0) represents a larger value of a measured value of an i-th photodetector and a measured value of zero, and an offset represents a value of a weight determined based on a measured value of each photodetector.

### Claim 3 (depends on 1)

The particle analysis system according to claim 1,
wherein the information processing unit calculates the fluorescence intensity by using an evaluation function represented by the following Formula (2)
[
x
1
⋮
x
n
]
=
(
[
S
T
]
[
L
]
[
S
]
)
-
1
[
S
T
]
[
L
]
[
y
1
⋮
y
m
]
⁢
L
=
[
λ
1
0
0
0
⋱
0
0
0
λ
m
]
,
λ
i
=
1
max
⁡
(
y
i
,
0
)
+
offset
(
2
)
in the formula, S represents a determinant indicating a shape of the single staining spectrum, ST represents a transposed determinant of S, L represents a determinant indicating the weight of the single staining spectrum, Xn (n=1 to the number of fluorescent dyes) represents a fluorescence intensity of an n-th fluorescent dye, ym (m=1 to the number of photodetectors) represents a measured value of an m-th photodetector, and an offset represents a value of a weight determined based on a measured value of each photodetector.

### Claim 4 (depends on 1)

The particle analysis system according to claim 1,
wherein the weight is a value calculated based on a measured value by autofluorescence of the particle detected by each photodetector.

### Claim 5 (depends on 1)

The particle analysis system according to claim 1,
wherein the weight is a value calculated based on a measured value by autofluorescence of the particle detected by each photodetector and the measured value by fluorescence of the particle labeled with the plurality of fluorescent dyes.

### Claim 6 (depends on 1)

The particle analysis system according to claim 1,
wherein the weight is a standard deviation value calculated based on an area obtained by integrating the measured values in each photodetector.

### Claim 7 (depends on 1)

The particle analysis system according to claim 1,
wherein the weight is an average value calculated based on a height indicating a peak value of the measured value in each photodetector.

### Claim 8 (depends on 1)

The particle analysis system according to claim 1, further including:
a sorter configured to sort particles that emit specific fluorescence based on a processing result by the information processing unit.

### Claim 9

A particle analysis method including:
acquiring light generated by irradiating a particle labeled with a plurality of fluorescent dyes with excitation light by a plurality of photodetectors; and
calculating fluorescence intensity of each fluorescent dye by performing separation processing on a measurement spectrum based on measured values from the plurality of photodetectors with a single staining spectrum of each fluorescent dye,
wherein the separation processing is performed by using a weighted least squares method (WLSM) including a weight determined based on a variation in the measured values.