Method of detecting bacterial/fungal contamination
OwnerJiayi HUANG, Scott Alan RICE +2inventors
InventorsJiayi HUANG, Scott Alan RICE, Stacy L. SPRINGS, Yie Hou LEE
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Abstract
The present invention relates to a method of detecting the presence of a bacterial and/or fungal cell in a sample through detecting the presence of nicotinamidase activity or nicotinamidase, wherein the presence of nicotinamidase activity or nicotinamidase indicates the presence of a bacterial and/or fungal cell in the sample. The invention also relates to a method for monitoring bacterial and/or fungal cell contamination in a cell of tissue culture comprising detecting the presence of nicotinamidase activity or nicotinamidase in the cell or tissue culture.
Claims16
A method for detecting the presence of a bacterial and/or fungal cell in a sample, the method comprising detecting the presence of nicotinamidase activity or nicotinamidase, wherein the presence of nicotinamidase activity or nicotinamidase indicates the presence of a bacterial and/or fungal cell in the sample.
A method for detecting bacterial and/or fungal cell contamination in a sample, the method comprising detecting the presence of nicotinamidase activity or nicotinamidase, wherein the presence of nicotinamidase activity or nicotinamidase indicates bacterial and/or fungal cell contamination in the sample.
A method for assessing the sterility of a sample, the method comprising detecting the presence of nicotinamidase activity or nicotinamidase in the sample, wherein the lack of nicotinamidase activity or nicotinamidase as compared to a reference indicates that the sample is sterile, sterilized or free from bacterial and/or fungal cell contamination.
A method for monitoring bacterial and/or fungal cell contamination in a cell or tissue culture, the method comprising detecting the presence of nicotinamidase activity or nicotinamidase in the cell or tissue culture, wherein the presence of nicotinamidase activity or nicotinamidase indicates bacterial and/or fungal cell contamination in the cell or tissue culture.
A kit for detecting the presence of a bacterial and/or fungal cell in a sample, the kit comprising determining the levels of nicotinic acid and nicotinamide in the sample, wherein a change in the ratio of nicotinic acid to nicotinamide levels as compared to a reference indicates the presence of the bacterial and/or fungal cell in the sample.
Dependencies are inferred from the claim text.