Process for the Production of Fucosylated Oligosaccharides
AssigneeChr. Hansen HMO GmbH
InventorsStefan Jennewein, Dirk Wartenberg, Katja Parschat
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Abstract
The present invention relates to a method for producing fucosylated oligosaccharides by using a recombinant prokaryotic host cell that is cultivated on a gluconeogenic substrate, as well as to the host cell and its use. The host cell is genetically modified in that the activity of a fructose-6-phosphate converting enzyme is abolished or lowered, and the transport of the produced fucosylated oligosaccharide through the cell membrane is facilitated by an exogenous transport protein.
Claims21
-25. (Cancelled)
A method for the production of fucosylated oligosaccharides using a genetically modified prokaryotic host cell, the method comprising:
- providing a prokaryotic host cell which has been genetically modified to have:
- (i) the fructose-6-phosphate pool in the cell increased by increasing the activity of a fructose-1,6-bisphosphate phosphatase;
- (ii) overexpression of at least one gene encoding an enzyme necessary for the de novo synthesis of GDP-fucose;
- and
- (iii) expression of an exogenous gene encoding an alpha-1,2-fucosyl-transferase and/or an alpha-1,3-fucosyltransferase;
- and
- cultivating said genetically modified prokaryotic host cell in a cultivation medium comprising at least one carbon and/or energy source selected from one or more of the group consisting of glycerol, succinate, malate, pyruvate, lactate, ethanol, and citrate;
- and
- adding lactose to the cultivation medium;
- wherein the fucosylated oligosaccharide is obtained from the medium in which the host cell is cultivated.
Dependencies are inferred from the claim text.