Method for producing tagatose from biomimetic silicon mineralized microcapsule immobilized multi-enzyme

Biomimetic silicon mineralized microcapsule immobilized multi-enzymes for producing tagatose, characterized in that the immobilized multi-enzymes are prepared by a method comprising the following steps:

• (1) pre-mixing the five enzymes involved in tagatose production:
• glucan phosphorylase, phosphoglucomutase, phosphoglucose isomerase, tagatose 6-phosphate 4-epimerase, and tagatose 6-phosphate phosphatase solutions, adding a solution of the above enzymes to a calcium chloride solution, pouring a sodium carbonate solution into the above solution, stirring, carrying out solid-liquid separation, and collecting a solid product, which is a calcium carbonate microsphere containing multi-enzymes;
• (2) mixing the calcium carbonate microsphere containing multi-enzymes with a solution of polyethyleneimine, carrying out solid-liquid separation, and collecting a solid product, which is a polyethyleneimine-calcium carbonate microsphere;
• (3) mixing the polyethyleneimine-calcium carbonate microsphere with a silicate solution, carrying out solid-liquid separation, and collecting a solid product, which is a biomimetic silicon mineralized calcium carbonate microsphere;
• and
• (4) mixing the biomimetic silicon mineralized calcium carbonate microsphere with ethylenediamine tetraacetic acid (EDTA), allowing a reaction to remove calcium carbonate, carrying out solid-liquid separation, and collecting a solid product, which is the biomimetic silicon mineralized microcapsule immobilized multi-enzymes.