# US 2024/0200008 A1 · Device for manufacturing cell-derived vesicles and manufacturing method using same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2024/0200008 A1 - Document ID: US20240200008A1 - Status: Published application - Kind: A1 - Application number: 18/593,346 - Filed: 2024-03-01 - Published: 2024-06-20 - Earliest claimed: 2021-09-02 - Assignees: Mdimune Inc. - Applicants: MDimune Inc. - Inventors: Jik Han JUNG; Byoung Jin KIM; Shin Gyu BAE - Web page: https://patentgrep.com/patent/US20240200008A1 ## CPC - C12M 23/58: {Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00)} - C12M 1/00: Apparatus for enzymology or microbiology - C12M 1/34: Measuring or testing with condition measuring or sensing means, e.g. colony counters - C12M 3/00: Tissue, human, animal or plant cell, or virus culture apparatus - C12M 23/38: {Caps; Covers; Plugs; Pouring means} - C12M 29/14: {Pressurized fluid} - C12M 29/24: {Recirculation of gas} - C12M 33/04: {by injection or suction, e.g. using pipettes, syringes, needles (pipettes in general B01L3/02)} - C12M 37/02: {Filters} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract The present invention relates to a method for manufacturing cell-derived vesicles using a depth filter. The present invention has the effect of enabling cell-derived vesicles to be manufactured stably, economically, and in large quantities through a simplified process. ## Claims ### Claim 1 A method for manufacturing cell-derived vesicles using a depth filter, comprising: a) extruding cells through a depth filter; and b) obtaining cell-derived vesicles from the extruded solution. ### Claim 2 (depends on 1) The method for manufacturing the cell-derived vesicles of claim 1, further comprising: before the extruding step of step a), a-1) harvesting the cells by passing a cell culture solution containing the cells through the depth filter; and a-2) resuspending the harvested cells by supplying a cell suspension, wherein the cells in step a) are cells in the cell suspension. ### Claim 3 (depends on 2) The method for manufacturing the cell-derived vesicles of claim 2, wherein the harvesting step, the resuspending step, and the extruding step are performed in one device. ### Claim 4 (depends on 2) The method for manufacturing the cell-derived vesicles of claim 2, wherein than the extruding step of step a) is performed at a higher pressure than the harvesting step of step a-1). ### Claim 5 (depends on 2) The method for manufacturing the cell-derived vesicles of claim 2, wherein the harvesting step of step a-1) is performed under pressure conditions of is 0.1 to 0.4 bar. ### Claim 6 (depends on 1) The method for manufacturing the cell-derived vesicles of claim 1, wherein the extruding step of step a) is performed under pressure conditions of 1 to 2.5 bar. ### Claim 7 (depends on 2) The method for manufacturing the cell-derived vesicles of claim 2, wherein the cell suspension is supplied in a reverse direction of the direction in which the cell culture solution is discharged from the depth filter. ### Claim 8 (depends on 1) The method for manufacturing the cell-derived vesicles of claim 1, wherein the extruding step of step a) is repeated 1 to 120 times. ### Claim 9 (depends on 8) The method for manufacturing the cell-derived vesicles of claim 8, wherein the repetition is performed by reciprocating through one depth filter. ### Claim 10 (depends on 8) The method for manufacturing the cell-derived vesicles of claim 8, wherein the repetition is performed by changing the pressure. ### Claim 11 (depends on 10) The method for manufacturing the cell-derived vesicles of claim 10, wherein the manufacturing method is performed by changing the pressure to 1 bar as a first pressure and 2.5 bar as a second pressure. ### Claim 12 (depends on 3) The method for manufacturing the cell-derived vesicles of claim 3, wherein the device comprises: a first pressure vessel and a second pressure vessel, of which a culture solution supply line to which a cell culture solution is supplied and a gas supply line are connected to one side; a first extrusion line connected to the other side of the first pressure vessel; a second extrusion line which is connected to the other side of the second pressure vessel and communicates with the first extrusion line; at least one depth filter which is disposed on the first extrusion line or the second extrusion line and through which the cell culture solution passes; and a harvesting line which communicates with the first extrusion line and the second extrusion line. ### Claim 13 (depends on 12) The method for manufacturing the cell-derived vesicles of claim 12, wherein at least one depth filter is disposed on the first extrusion line and the second extrusion line, respectively. ### Claim 14 (depends on 13) The method for manufacturing the cell-derived vesicles of claim 13, wherein the device for manufacturing the cell-derived vesicles comprises: a first filter assembly and a second filter assembly, on one side of which a culture solution supply line to which a cell culture solution is supplied and a gas supply line are connected, and in which a depth filter is provided; a first extrusion line connected to the other side of the first filter assembly; a second extrusion line which is connected to the other side of the second filter assembly and communicates with the first extrusion line; and a harvesting line which communicates with the first extrusion line and the second extrusion line. ### Claim 15 (depends on 14) The method for manufacturing the cell-derived vesicles of claim 14, wherein the first filter assembly and the second filter assembly comprise: a lower portion in which at least one mounting hole mounted with the depth filter is formed; a middle portion which is formed so that the depth filter is contained therein and coupled to the lower portion; and an upper portion which is fastened to the upper end of the middle portion and seals the inside together with the lower portion and the middle portion. ### Claim 16 (depends on 15) The method for manufacturing the cell-derived vesicles of claim 15, wherein the first filter assembly and the second filter assembly further comprise: a cap which is provided to be detachable from the mounting hole and seals the mounting hole while inserted into the mounting hole. ### Claim 17 A method for manufacturing cell-derived vesicles with reduced risk of exposure to external contamination comprising: a) harvesting cells by passing a cell culture solution containing the cells through a depth filter; b) resuspending the harvested cells by supplying a cell suspension; c) extruding the cells resuspended in step b) through a depth filter; and d) obtaining cell-derived vesicles from the extruded solution, wherein steps a) to d) are performed in one device. ### Claim 18 (depends on 17) The method for manufacturing the cell-derived vesicles of claim 17, wherein the manufacturing method is performed in one device to reduce the risk of cells or cell-derived vesicles to be exposed to an external environment to be contaminated or deformed. ### Claim 19 (depends on 17) The method for manufacturing the cell-derived vesicles of claim 17, wherein the manufacturing method is performed in one device to improve a process rate. ### Claim 20 (depends on 17) The method for manufacturing the cell-derived vesicles of claim 17, wherein the device comprises: a first pressure vessel and a second pressure vessel, of which a culture solution supply line to which a cell culture solution is supplied and a gas supply line are connected to one side; a first extrusion line connected to the other side of the first pressure vessel; a second extrusion line which is connected to the other side of the second pressure vessel and communicates with the first extrusion line; at least one depth filter which is disposed on the first extrusion line or the second extrusion line and through which the cell culture solution passes; and a harvesting line which communicates with the first extrusion line and the second extrusion line.