# US 2024/0200006 A1 · Microfluidic control

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- Number: US 2024/0200006 A1
- Document ID: US20240200006A1
- Status: Published application
- Kind: A1
- Application number: 18/591,786
- Filed: 2024-02-29
- Published: 2024-06-20
- Earliest claimed: 2018-04-03
- Assignees: Emulate, Inc.
- Applicants: EMULATE, Inc.
- Inventors: Christopher David Hinojosa; Grace Ahn
- Web page: https://patentgrep.com/patent/US20240200006A1

## CPC

- C12M 23/16: {Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093)}
- F04B 19/006: {Micropumps (F04B43/043 and F04B43/095 take precedence)}
- B01L 3/505: {Flexible containers without fluid transport within}
- C12M 23/26: {flexible (flexible containers for laboratory use B01L3/505)}
- C12M 25/02: {Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00)}
- C12M 29/00: {Means for introduction, extraction or recirculation of materials, e.g. pumps (pumps per se F04B)}
- C12M 41/18: {Heat exchange systems, e.g. heat jackets or outer envelopes}
- F04B 23/02: having reservoirs
- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- B01L 3/50273: {characterised by the means or forces applied to move the fluids}
- B01L 2400/0481: squeezing of channels or chambers
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- F04B: POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS (machines for liquids, or pumps, of rotary-piston or oscillating-piston type F04C; non-positive-displacement pumps F04D; pumping of fluid by direct contact of another fluid or by using inertia of fluid to be pumped F04F)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

## Abstract

The present invention is related to the field of fluidic devices, and in particular, microfluidic cell culture systems. The present invention provides pumping, recirculation and sampling for a microfluidic device or devices. The present invention provides solutions to the control of microfluidics for both terrestrial and space applications, including the control over the movement of fluids in zero gravity or microgravity.

## Claims

### Claim at position 1

1-174. (canceled)

### Claim 175

An assembly, comprising a) one or more flexible cell culture media reservoirs and b) one or more flexible reagent reservoirs contained within c) a container, said container i) in fluidic communication with d) a pump and e) a working fluid reservoir and ii) comprising a working fluid, said flexible cell culture reservoirs i) in fluidic communication with d) one or more microfluidic devices and ii) comprising cell culture media, said one or more flexible reagent reservoirs i) in fluidic communication with one or more microfluidic devices and ii) comprising reagent, wherein there is no mixing of said working fluid with said cell culture media.

### Claim 176 (depends on 175)

The assembly of claim 175, wherein said container is rigid or flexible.

### Claim 177 (depends on 175)

The assembly of claim 175, wherein said one or more microfluidic devices are outside said container.

### Claim 178 (depends on 177)

The assembly of claim 177, wherein said one or more microfluidic devices are housed in a manifold.

### Claim 179 (depends on 175)

The assembly of claim 175, wherein said one or more microfluidic devices comprise living cells on a surface.

### Claim 180 (depends on 175)

The assembly of claim 175, further comprising valves to control the flow of said culture media out of said flexible reservoirs.

### Claim 181 (depends on 175)

The assembly of claim 175, wherein said reagent is selected from the group consisting of a lysate reagent, a fixative reagent and a staining reagent.