# US 2024/0197616 A1 · Cosmetic composition comprising chlorella protothecoides extract for skin whitening or wrinkle improvement and preparation method therefor

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- Number: US 2024/0197616 A1
- Document ID: US20240197616A1
- Status: Published application
- Kind: A1
- Application number: 18/287,237
- Filed: 2022-04-25
- Published: 2024-06-20
- Earliest claimed: 2021-04-26
- Assignees: Daebong Ls, Ltd; Daesang Corporation
- Applicants: Daebong Ls Co., Ltd.; Daesang Corporation
- Inventors: Wang-Bae KIM; Dong-Joong IM; Keon-Woo KIM; Jin-Oh PARK; Bo-Ra KIM; Ji-Eun HWANG
- Web page: https://patentgrep.com/patent/US20240197616A1

## CPC

- A61K 8/9722: Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
- A61K 8/64: Proteins; Peptides; Derivatives or degradation products thereof
- A61K 8/645: {Proteins of vegetable origin; Derivatives or degradation products thereof}
- A61K 8/9789: Magnoliopsida [dicotyledons]
- A61Q 19/02: for chemically bleaching or whitening the skin
- A61Q 19/08: Anti-ageing preparations
- A61K 2800/80: Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS

## Abstract

A composition contains a Chlorella protothecoides extract. The composition can be used for skin whitening or wrinkle improvement. The composition containing the Chlorella protothecoides extract may be widely used as a cosmetic for skin whitening or wrinkle improvement since the problem of limited application as a cosmetic composition due to the characteristic fishy smell and dark color of the composition using Chlorella has been resolved.

## Claims

### Claim at position 1

1-4. (canceled)

### Claim 5

A method for preparing a Chlorella protothecoides extract, the method comprising:
obtaining a dispersion by dispersing Chlorella protothecoides in a solvent;
obtaining a reaction solution by adding a protease to the dispersion; and
obtaining an extract comprising a low-molecular-weight peptide from the reaction solution.

### Claim 6 (depends on 5)

The method according to claim 5, wherein the dispersion is achieved by compressing at 100-5000 psi.

### Claim 7 (depends on 5)

The method according to claim 5, wherein 10-150 parts by weight of the protease is added based on 100 parts by weight of the Chlorella protothecoides.

### Claim 8 (depends on 5)

The method according to claim 5, further comprising obtaining a fraction by fractionating the extract with an ultrafiltration membrane.

### Claim 9

A method for skin whitening or wrinkle improvement, the method comprising:
applying a cosmetic composition comprising a Chlorella protothecoides extract to a subject in need thereof.

### Claim 10 (depends on 9)

The method of claim 9, wherein the Chlorella protothecoides extract comprises a low-molecular-weight peptide.

### Claim 11 (depends on 10)

The method of claim 10, wherein the low-molecular-weight peptide is obtained using a protease.

### Claim 12 (depends on 11)

The method of claim 11, wherein, wherein the protease is derived from papaya.