# US 2024/0197294 A2 · Compositions, devices and methods for testing inanimate surfaces, groups and individuals for pathogen infection > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2024/0197294 A2 - Document ID: US20240197294A2 - Status: Published application - Kind: A2 - Application number: 17/927,345 - Filed: 2021-05-26 - Published: 2024-06-20 - Earliest claimed: 2020-05-26 - Assignees: 3M Innovative Properties Company - Applicants: 3M Innovative Properties Company - Inventors: Michael Wohl - Web page: https://patentgrep.com/patent/US20240197294A2 ## CPC - A61B 10/00: Instruments for taking body samples for diagnostic purposes (for taking samples of blood A61B5/15); Other methods or instruments for diagnosis, e.g. for vaccination diagnosis, sex determination or ovulation-period determination; Throat striking implements - A61B 10/0045: {Devices for taking samples of body liquids} - G01N 33/497: of gaseous biological material, e.g. breath - A61B 2010/0006: {involving a colour change} - A61B 2010/0087: {Breath samples} - G01N 2333/165: Coronaviridae, e.g. avian infectious bronchitis virus - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Disclosed herein are compositions, devices and methods for detecting analytes in air samples obtained from a subject. Compositions can be included on inanimate surfaces such as masks to be worn by an individual having a test substrate for capturing analytes. ## Claims ### Claim 1 A mask insert comprising a first layer, a test substrate for capturing an analyte in an air sample obtained from a subject, and a second layer, wherein the first layer and the second layer form an outer layer substantially surrounding the test substrate, wherein overlapping regions of the first layer and the second layer are bonded, wherein at least a portion of the test substrate is bonded to at least one of the first layer and the second layer, and wherein the test substrate is configured to be separated from at least a portion of the outer layer. ### Claim 2 (depends on 1) The mask insert of claim 1, further comprising a fastener on a surface of at least one of the first layer or the second layer and configured to couple the mask insert to a surface of a mask. ### Claim 3 (depends on 1) The mask insert of claim 1, further comprising a perforation in the outer layer proximate to an end of the mask insert. ### Claim 4 (depends on 1) The mask insert of claim 1, further comprising a notch coextensive with the perforation. ### Claim 5 (depends on 1) The mask insert of claim 1, wherein the test substrate comprises a material selected from the group consisting of synthetic fibers, natural fibers, and combinations thereof. ### Claim 6 (depends on 5) The mask insert of claim 5, wherein the test substrate comprises a material selected from the group consisting of hydrophobic fibers, hydrophilic fibers, and combinations thereof. ### Claim 7 (depends on 6) The mask insert of claim 6, wherein the hydrophobic fibers are selected from the group consisting of polypropylene, polylactone, poly(caprolactone), poly (L-lactic acid), poly (glycolic acid), co-poly(alkyl acrylate), polybutadiene, polyethylene, polystyrene, polyacrylonitrile, polyethylene (terephthalate), polysulfone, polycarbonate, poly(vinyl chloride), and combinations thereof. ### Claim 8 (depends on 6) The mask insert of claim 6, wherein the hydrophilic fibers are selected from the group consisting of linear poly(ethylenimine), cellulose, cellulose acetate and other grafted cellulosics, poly (hydroxyethylmethacrylate), poly (ethyleneoxide), polyvinylpyrrolidone, poly(acrylic acid), poly(ethylene glycol), poly(vinyl alcohol), poly (vinyl acetate), poly(acrylamide), proteins, poly (vinyl pyrrolidone), poly(styrene sulfonate), and combinations thereof. ### Claim 9 (depends on 5) The mask insert of claim 5, wherein the test substrate comprises a material selected from the group consisting of acrylonitrile/butadiene copolymer, cellulose, cellulose acetate, chitosan, collagen, DNA, fibrinogen, fibronectin, nylon, poly(acrylic acid), poly(chloro styrene), poly(dimethyl siloxane), poly(ether imide), poly(ether sulfone), poly(ethyl acrylate), poly(ethyl vinyl acetate), poly(ethyl-co-vinyl acetate), poly(ethylene oxide), poly(ethylene terephthalate), poly(lactic acid-co-glycolic acid), poly(methacrylic acid) salt, poly(methyl methacrylate), poly(methyl styrene), poly(styrene sulfonic acid) salt, poly(styrene sulfonyl fluoride), poly(styrene-co-acrylonitrile), poly(styrene-co-butadiene), poly(styrene-co-divinyl benzene), poly(vinyl acetate), poly(vinyl alcohol), poly(vinyl chloride), poly(vinylidene fluoride), polyacrylamide, polyacrylonitrile, polyamic acid (PAA), polyamide, polyaniline, polybenzimidazole, polycaprolactone, polycarbonate, polydimethylsiloxane-co-polyethyleneoxide, polyetheretherketone, polyethylene, polyethyleneimine, polyimide, polyisoprene, polylactide, polypropylene, polystyrene, polysulfone, polyurethane, polyvinylpyrrolidone, proteins, SEBS copolymer, silk, styrene/isoprene copolymer, poly(vinylidene fluoride)-blend-poly(methyl methacrylate), polystyrene-blend-poly(vinylmethylether), poly(methyl methacrylate)-blend-poly(ethyleneoxide), poly(hydroxypropyl methacrylate)-blend poly(vinylpyrrolidone), poly(hydroxybutyrate)-blend-poly(ethylene oxide), protein blend-polyethyleneoxide, polylactide-blend-polyvinylpyrrolidone, polystyrene-blend-polyester, polyester-blend-poly(hyroxyethyl methacrylate), poly(ethylene oxide)-blend poly(methyl methacrylate), poly(hydroxystyrene)-blend-poly(ethylene oxide), and combinations thereof. ### Claim 10 (depends on 1) The mask insert of claim 1, wherein the test substrate comprises electret. ### Claim 11 (depends on 1) The mask insert of claim 1, wherein the first layer and the second layer comprise synthetic fibers, natural fibers, and combinations thereof. ### Claim 12 (depends on 1) The mask insert of claim 1, wherein the bonding substantially reduces air flow through the bonded regions of the mask insert. ### Claim 13 (depends on 1) The mask insert of claim 1, further comprising an assay selected from the group consisting of a vertical flow assay and a lateral flow assay. ### Claim 14 (depends on 1) The mask insert of claim 1, further comprising a buffer dispensing device. ### Claim 15 A system for detecting an analyte in an air sample obtained from a subject, the system comprising: a mask insert, the mask insert comprising: a first layer, a test substrate for capturing an analyte in an air sample obtained from a subject, a second layer, wherein the first layer and the second layer form an outer layer substantially surrounding the test substrate, wherein overlapping regions of the first layer and the second layer are bonded, wherein at least a portion of the test substrate is bonded to at least one of the first layer and the second layer, and wherein the test substrate is configured to be separated from at least a portion of the outer layer; and a mask worn by the subject, wherein the mask insert is coupled to a surface of the mask. ### Claim 16 (depends on 15) The system of claim 15, wherein the mask insert is coupled with one of a vertical flow assay and a lateral flow assay. ### Claim 17 (depends on 15) The system of claim 15, wherein the mask insert further comprises a buffer dispensing device. ### Claim 18 (depends on 15) The system of claim 15, wherein the test substrate comprises synthetic fibers, natural fibers, and combinations thereof. ### Claim 19 (depends on 16) The system of claim 16, wherein the test substrate comprises electret. ### Claim 20 (depends on 15) The system of claim 15, wherein the first layer and the second layer comprise synthetic fibers, natural fibers, and combinations thereof.