# US 2024/0197253 A1 · Microneedle biosensor and manufacturing method for same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2024/0197253 A1 - Document ID: US20240197253A1 - Status: Published application - Kind: A1 - Application number: 18/287,536 - Filed: 2021-12-16 - Published: 2024-06-20 - Earliest claimed: 2021-04-22 - Assignees: Albiti Inc. - Applicants: Albiti Inc. - Inventors: JUN YOUNG AHN; Eun Hee JANG - Web page: https://patentgrep.com/patent/US20240197253A1 ## CPC - A61B 5/685: {Microneedles} - A61B 5/1451: {for interstitial fluid} - A61B 5/14532: {for measuring glucose, e.g. by tissue impedance measurement} - A61B 5/14865: {invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors} - C23C 14/025: {Metallic sublayers} - C23C 14/042: {using masks} - C23C 14/205: {by cathodic sputtering} - C25D 3/50: of platinum group metals - C25D 7/00: Electroplating characterised by the article coated - G01N 27/327: Biochemical electrodes {, e.g. electrical or mechanical details for in vitro measurements} - A61B 5/1473: invasive, e.g. introduced into the body by a catheter - A61B 2562/0209: Special features of electrodes classified in A61B5/24, A61B5/25, A61B5/283, A61B5/291, A61B5/296, A61B5/053 - A61B 2562/0215: Silver or silver chloride containing - A61B 2562/028: Microscale sensors, e.g. electromechanical sensors [MEMS] - A61B 2562/046: in a matrix array - A61B 2562/125: characterised by the manufacture of electrodes - A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D) - C23C: COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL (making metal-coated products by extrusion B21C23/22; covering with metal by connecting pre-existing layers to articles, see the relevant places, e.g. B21D39/00, B23K; metallising of glass C03C; metallising mortars, concrete, artificial stone, ceramics or natural stone C04B41/00; enamelling of, or applying a vitreous layer to, metals C23D; treating metal surfaces or coating of metals by electrolysis or electrophoresis C25D; single-crystal film growth C30B; by metallising textiles D06M11/83; decorating textiles by locally metallising D06Q1/04) - C25D: PROCESSES FOR THE ELECTROLYTIC OR ELECTROPHORETIC PRODUCTION OF COATINGS; ELECTROFORMING; APPARATUS THEREFOR - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract Disclosed is a microneedle biosensor and a manufacturing method for same, the microneedle biosensor comprising: a working electrode that comprises a circular thin-film first base, a plurality of microneedles protruding vertically upwards from the first base, and first wiring extending from one side of the circumference of the first base; a counter electrode that comprises a second base which is a three-quarter circle strip thin-film that is concentric with the first base and separated by a set distance from the circumference of the first base, a plurality of microneedles protruding vertically upwards from the second base, and second wiring extending from one side of the second base so as to be disposed on a level with the first wiring; and a reference electrode that comprises a third base which is a quarter circle strip thin-film that is separated by a set difference from the other side of the second base, is concentric with the first base and is separated by a set distance from the circumference of the first base, a plurality of microneedles protruding vertically upwards from the third base, and third wiring extending from one side of the third base. ## Claims ### Claim 1 A microneedle biosensor comprising: a working electrode including a first base of a circular thin film type, a plurality of microneedles which perpendicularly protrudes on the first base, and a first wiring line which extends from one end of a circumference of the first base; a counter electrode which includes a second base of a strip thin film type which forms a part of a second circumference spaced apart from the circumference of the first base with a setting distance to be concentric with the first base, a plurality of microneedles which perpendicularly protrudes on the second base, and a second wiring line which extends from one end of the second base to be horizontally disposed with the first wiring line; and a reference electrode which includes a third base of a strip thin film type which is spaced apart from the other end of the second base with a setting interval and forms the second circumference with the strip shape of the second base, a plurality of microneedles which perpendicularly protrudes on the third base, and a third wiring line which extends from one end of the third base. ### Claim 2 (depends on 1) The microneedle biosensor of claim 1, wherein the second base occupies ¾ of the second circumference and the third base occupies ¼ of the second circumference. ### Claim 3 (depends on 1) The microneedle biosensor of claim 1, wherein the first wiring line perpendicularly extends from one end of the circumference of the first base, the second wiring line extends from one end of the second base to be horizontally disposed with the first wiring line, and the third wiring line extends from one end of the third base so as to be horizontally disposed with the first wiring line. ### Claim 4 A manufacturing method of a microneedle biosensor, comprising: a) forming a mold by forming grooves corresponding to shapes of microneedles of each of a working electrode, a counter electrode, and a reference electrode in a solid resin block; b) imprinting a polymer layer of each of the working electrode, the counter electrode, and the reference electrode on the mold using polymer; c) forming a metal layer by forming shadow masks corresponding to patterns of the working electrode, the counter electrode, and the reference electrode on the polymer layer and sputtering Au or Au+Ti/Cr adhesive layer; and d) forming a passivation layer on the metal layer. ### Claim 5 (depends on 4) The manufacturing method of a microneedle biosensor of claim 4, further comprising: after performing the step d), plating with Pt-black and coating with nafion on tips of the microneedles of the working electrode. ### Claim 6 (depends on 4) The manufacturing method of a microneedle biosensor of claim 4, further comprising: after performing the step d), coating the microneedles of the reference electrode with Ag/AgCl. ### Claim 7 (depends on 4) The manufacturing method of a microneedle biosensor of claim 4, wherein the solid resin block is a polydimethylsiloxane (PDMS) or polytetrafluoroethylene (PTFE) material. ### Claim 8 (depends on 4) The manufacturing method of a microneedle biosensor of claim 4, wherein in the step b), the polymer is acryl or PLA. ### Claim 9 (depends on 4) The manufacturing method of a microneedle biosensor of claim 4, wherein in the step c), in the patterns of the working electrode, the counter electrode, and the reference electrode, the working electrode includes a first base of a circular thin film type, the counter electrode includes a second base of a strip thin film type which forms a part of a second circumference spaced apart from a circumference of the first base with a setting distance to be concentric with the first base, and the reference electrode includes a third base of a strip thin film type which is spaced apart from the other end of the second base with a setting interval and forms the second circumference together with the strip shape of the second base.