Brassica intergeneric crossbred plant containing large amount of glucoraphanin, and creation method therefor
AssigneesNational Agriculture and Food Research Organization, Kagome Co., Ltd.
InventorsTomohiro KAKIZAKI, Takayoshi OHARA, Etsuko ITABASHI, Mitsuyo KAWASAKI, Ryota ENDO, Yusuke USHIDA
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Abstract
A Brassicaceae plant containing a large amount of glucoraphanin is obtained. A first parent plant and a second parent plant, which are both Brassicaceae plants classified into different genera, are crossbred, thereby obtaining an intergeneric crossbred plant. The first parent plant has 5 mg/100 g (fresh weight) or more of glucoraphanin. The second parent plant has a loss-of-function type glucoraphasatin synthase gene.
Claims21
An intergeneric crossbred plant from a Brassica plant and a Raphanus plant, for which a ratio of glucoraphanin content to glucoraphenin content is 1.0 or more.
An intergeneric crossbred plant from a Brassica plant and a Raphanus plant, which comprises a loss-of-function type glucoraphasatin synthase gene.
A method for creating a Brassicaceae intergeneric crossbred plant, the method comprising:
- a step of crossbreeding a first parent plant and a second parent plant;
- and
- a step of obtaining an intergeneric crossbred plant from the first parent plant and the second parent plant, wherein
- the first parent plant and the second parent plant are Brassicaceae plants of different genera,
- the first parent plant comprises 5 mg/100 g (fresh weight) or more of glucoraphanin, and the second parent plant comprises a loss-of-function type glucoraphasatin synthase gene.
A method for allowing a Brassicaceae plant to have a large amount of glucoraphanin, the method comprising:
- a first step of preparing a Brassicaceae plant comprising 5 mg/100 g (fresh weight) or more of glucoraphanin as a first parent plant;
- a second step of preparing a Brassicaceae plant of a genus different from the first parent plant, in which the function of glucoraphasatin synthase is deficient or reduced, as a second parent plant;
- and
- a third step of crossbreeding the first parent plant and the second parent plant.
Dependencies are inferred from the claim text.