Light-emitting device and manufacturing method thereof, and display substrate

A manufacturing method of a light-emitting device, comprising:

• forming a first electrode, a second electrode and a light-emitting functional layer;
• wherein the first electrode and the second electrode are arranged opposite to each other, and the light-emitting functional layer is located between the first electrode and the second electrode;
• wherein
• the light-emitting functional layer includes a first light-emitting layer, and the first light-emitting layer includes a first light-emitting material;
• the first light-emitting material is configured to emit light in response to a control of an electrical signal on the first electrode and an electrical signal on the second electrode;
• and
• the light-emitting functional layer further includes a second light-emitting material;
• the second light-emitting material is configured to absorb light that does not exit from the light-emitting device in the light emitted from the first light-emitting material, and to emit light.