Method for calibrating a data set of a target analyte

A method for reducing inter- and intra-instrument signal variations of a data set of a target analyte in a sample comprising:

• (a) providing a normalization coefficient for calibrating the data set by using a reference value, a reference cycle and the data set;
• wherein the data set is obtained from a signal-generating process for the target analyte using a signal-generating means;
• wherein the data set comprises a plurality of data points comprising cycles of the signal-generating process and signal values at the cycles;
• wherein the cycle is a unit of reaction time;
• wherein step (a) comprises the following steps:
• performing the signal-generating process on the sample;
• obtaining the data set via by the signal-generating process;
• selecting the reference cycle from the cycles of the data set;
• and
• determining a signal value at the reference cycle;
• wherein the reference value is a value arbitrarily determined from a real number except zero;
• providing the normalization coefficient by defining a relationship between the reference value and a signal value at a cycle of the data set corresponding to the reference cycle;
• wherein the relationship between the signal value at the cycle of the data set corresponding to the reference cycle and the reference value is a difference between the signal value at the cycle of the data set corresponding to the reference cycle and the reference value;
• and
• (b) providing a calibrated data set by obtaining calibrated signal values by applying the normalization coefficient to the signal values of the data set.

A non-transitory computer-readable storage medium containing instructions to configure a processor to perform a method for reducing inter- and intra-instrument signal variations of a data set of a target analyte in a sample, the method comprising:

• (a) providing a normalization coefficient for calibrating the data set by using a reference value, a reference cycle and the data set;
• wherein the data set is obtained from a signal-generating process for the target analyte using a signal-generating means;
• wherein the data set comprises a plurality of data points comprising cycles of the signal-generating process and signal values at the cycles;
• wherein the cycle is a unit of reaction time;
• wherein step (a) comprises the following steps:
• performing the signal-generating process on the sample;
• obtaining the data set via by the signal-generating process;
• selecting the reference cycle from the cycles of the data set;
• and
• determining a signal value at the reference cycle;
• wherein the reference value is a value arbitrarily determined from a real number except zero;
• providing the normalization coefficient by defining a relationship between the reference value and a signal value at a cycle of the data set corresponding to the reference cycle;
• wherein the relationship between the signal value at the cycle of the data set corresponding to the reference cycle and the reference value is a difference between the signal value at the cycle of the data set corresponding to the reference cycle and the reference value;
• and
• (b) providing a calibrated data set by obtaining calibrated signal values by applying the normalization coefficient to the signal values of the data set.