Detection of biomarkers on vesicles for the diagnosis and prognosis of diseases and disorders

A method comprising:

• a) providing a biological sample comprising vesicles;
• b) contacting a solid support comprising capture agents associated therewith with the biological sample under conditions wherein the capture agents selectively bind to a first biomarker, if present, on the vesicles, thereby capturing said vesicles having said first biomarker on the solid support;
• c) separating the solid support from the biological sample;
• and
• d) detecting a second biomarker on the vesicles captured on the solid support using a detection agent that selectively binds to the second biomarker, wherein the vesicles are not lysed or permeabilized.