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US 2024/0192213 A1Published application
Method of detecting disease related biomarkers in bodily fluid sample
AssigneeThe Administrators of the Tulane Educational Fund
InventorsYe Tony HU, Bo NING, Yating XIAO
Life of this patent
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Abstract
Method and system for detecting disease in blood sample is described. By extracting extracellular vesicles (EVs) from the blood sample and employing liposome fusion probes, it is shown that the test can be completed within hours instead of weeks, and the detection limit can be significantly lowered.
Claims16
A method of detecting the presence of a disease-specific protein in a bodily fluid sample, comprising the steps of:
- a) extracting extracellular vesicles (EVs) in the bodily fluid sample by use of a first antibody against the disease-specific protein;
- b) mixing liposome fusion probes with the EVs in step a), wherein said liposome fusion probes contain RT, recombinase polymerase amplification (RPA), and CRISPR/Cas12a reagents;
- and
- c) detecting the presence of the disease-specific protein using a fluorescent detection system.
1 more element
A diagnostic kit for detecting the presence of a disease-specific protein in a bodily fluid sample, comprising:
- a) a first antibody against the disease-specific protein;
- b) liposome fusion probes, wherein said liposome fusion probes contain RT, recombinase polymerase amplification (RPA), and CRISPR/Cas12a reagents;
- and
- c) a fluorescent detection system.
1 more element
Dependencies are inferred from the claim text.