Kinetic immunoassay systems and methods

A method for analyzing a sample, the method comprising:

• mixing a first plurality of beads with the sample to form a mixture in a reactor of a microfluidic chip, wherein:
• the sample comprises a plurality of analytes,
• each bead of the first plurality of beads is coupled to a plurality of affinity reagents, and
• each affinity reagent of the plurality of affinity reagents is configured to bind to the analyte;
• binding a first subset of the plurality of analytes to a first subset of the plurality of affinity reagents coupled to a first bead of the first plurality of beads in the reactor;
• binding a second subset of the plurality of analytes to a second subset of the plurality of affinity reagents coupled to a second bead of the first plurality of beads in the reactor:
• coupling a first subset of a plurality of label compounds to the first bead, wherein the plurality of label compounds is configured to bind to the analyte;
• coupling a second subset of the plurality of label compounds to the second bead;
• flowing a first portion of the mixture from the reactor through a first fluidic path defined by a plurality of structures in the microfluidic chip, wherein:
• the first portion of the mixture comprises the first bead coupled to the first subset of the plurality of label compounds;
• flowing a second portion of the mixture from the reactor through the first fluidic path after flowing the first portion of the mixture through the first fluidic path, wherein:
• the second portion of the mixture comprises the second bead coupled to the second subset of the plurality of label compounds;
• flowing a solution through the microfluidic chip, such that the solution intersects the first fluidic path;
• contacting the first portion of the mixture to the solution;
• measuring a first amount of the first subset of the plurality of label compounds after contacting the first portion of the mixture to the solution;
• contacting the second portion of the mixture to the solution after contacting the first portion of the mixture to the solution;
• and
• measuring a second amount of the second subset of the plurality of label compounds after measuring the first amount.