Biomimetic liposomes and methods of making and using the same
AssigneeWorcester Polytechnic Institute
InventorsChristina M. Bailey-Hytholt, Daniel Zimmer
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Abstract
This disclosure relates generally to biomimetic proteoliposomes, a method of making and a method of using the same. In particular, this disclosure provides proteoliposomes comprising one or more phospholipid carrier and one or more protein embedded in the one or more phospholipid carrier, wherein the one or more phospholipid carrier comprises a phospholipid composition with similar proportions of phospholipids as a naturally occurring cell type and a phospholipid concentration of about 1-50 mM; and wherein the one or more protein comprises a protein composition with similar proportions of proteins as the naturally occurring cell type.
Claims40
A proteoliposome comprising:
- one or more phospholipid carrier and one or more protein embedded in the one or more phospholipid carrier;
- wherein the one or more phospholipid carrier comprises a phospholipid composition with similar proportions of phospholipids as a naturally occurring cell type and a phospholipid concentration of about 1-50 mM;
- and
- wherein the one or more protein comprises a protein composition with similar proportions of proteins as the naturally occurring cell type.
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A method comprising:
- (a) a first mixing of one or more phospholipid to form a phospholipid carrier solution;
- and
- (b) a second mixing of the phospholipid carrier solution with a protein solution to produce one or more proteoliposome, wherein the protein solution comprises one or more protein.
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A method for screening pharmaceuticals, comprising:
- (a) incubating one or more of a placental proteoliposome (PPL) and a PPL-ECM with one or more molecule of interest to form an incubation product;
- (b) filtering the incubation product to produce a filtered product;
- and
- (c) quantifying the filtered product to assess transport of the one or more molecule of interest into one or more of the PPL and the PPL-ECM.
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Dependencies are inferred from the claim text.