Systems and methods for detection
AssigneePhotoswitch Biosciences, Inc.
InventorsAndrew Blatz, Thomas Lila, Jay Trautman
Life of this patent
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Abstract
Provided herein are devices, systems, and methods for detection of sensors, such as light-detectable sensors. The devices or systems as described herein may comprise an illumination assembly configured to direct a light to at least a well of a plurality of wells in the array of wells and a detection assembly configured to detect a signal from the well.
Claims11
1-82. (canceled)
A method of screening a biological activity of a molecule or salt thereof in a plurality of cells, the method comprising:
- (a) adding the molecule or salt thereof to at least a portion of a plurality of wells, wherein a well of the plurality of wells comprises at least one cell of the plurality of cells;
- and
- (b) detecting a presence or an absence of activation of a sensor within each well of the plurality of wells, wherein the detecting occurs substantially in parallel, and wherein a sampling rate across the plurality of wells is greater than about 100 Hz.
A method of screening a biological activity of a molecule or salt thereof in a plurality of cells, the method comprising:
- (a) providing an array of wells comprising a plurality of wells, wherein each well of the plurality of wells comprises a cell of the plurality of cells, wherein at least a portion of the plurality of cells comprise a sensor;
- (b) adding the molecule or salt thereof to at least a portion of the plurality of wells;
- (c) directing a light from an excitation source to each well of the plurality of wells to illuminate at least a portion of each well of the plurality of wells to form an at least partially illuminated well;
- and
- (d) detecting a presence or an absence of activation of the sensor within each well of the plurality of wells, wherein the detecting occurs substantially in parallel, and wherein a sampling rate across the plurality of wells is greater than about 100 Hz.
A system comprising an illumination assembly and a detection assembly, wherein the system is configured to:
- (a) direct light to a plurality of wells of an array of wells;
- (b) collect, substantially in parallel, a signal from each well of the plurality of wells;
- and
- (c) transfer each signal, substantially in parallel, to a corresponding detector, wherein the system has a sampling rate of the signal across the plurality of wells that is greater than about 100 Hertz (Hz).
Dependencies are inferred from the claim text.