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US 2024/0191298 A1Published application
Dna-barcoded antigen multimers and method of use thereof
AssigneeBoard of Regents, The University of Texas System
InventorsNing JIANG, Shuqi ZHANG, Keyue MA, Chenfeng HE, Alexandra A. SCHONNESEN
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Abstract
Provided herein are methods compositions and methods to generate pMHC libraries, and methods of using the pMHC libraries to determine the sequences of T cell receptors, and T cell developmental and activation status.
Claims17
A method for producing a DNA-barcoded peptide multimer comprising:
- (a) performing in vitro transcription/translation (IVTT) on a peptide-encoding oligonucleotide to obtain a library of peptides;
- (b) contacting the library of peptides with biotinylated MHC monomers comprising UV-cleavable peptides;
- (c) applying UV light to exchange the UV-cleavable peptides on the MHC monomers with the library peptides to form peptide MHC monomers (pMHCs);
- (d) combining the pMHCs with a multimer backbone comprising streptavidin, wherein streptavidin is linked to an oligonucleotide comprising the peptide-encoding oligonucleotide via a DNA handle comprising a molecular identifier (MID) to produce a DNA-barcoded peptide multimer.
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Dependencies are inferred from the claim text.