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US 2024/0191293 A1Published application
Compositions and methods for simultaneous genetic analysis of multiple libraries
AssigneeResolution Bioscience, Inc.
InventorsJiannan Guo, Carolyn Hutcheon, Mark Li, Lee Lim, Ira Pekker, Chen-Hsun Tsai
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Abstract
The disclosure provides compositions and methods that combine the benefits of different genetic analysis methods by performing simultaneous genetic analysis on a single pool of different libraries that were generated for different approaches, such as a combined library comprising a LPWG library and a target-enriched library.
Claims20
A kit comprising one or more capture probe modules,
- wherein each capture probe module comprises a tail sequence and a capture probe sequence capable of hybridizing to a target sequence in a test sample;
- wherein the one or more capture probe modules have passed a probe Quality Control (QC) process.
A kit comprising a set of adaptors,
- wherein each adaptor comprises an adaptor module;
- wherein the set of adaptors have passed an adaptor Quality Control (QC) process.
A kit comprising:
- (a) a set of adaptors, wherein each adaptor comprises an adaptor module comprising an amplification region;
- (b) one or more capture probe modules, wherein each capture probe module comprises a tail sequence and a capture probe sequence capable of hybridizing to a target sequence in a test sample, wherein the tail sequence of each capture probe module comprises a Library Tag;
- (c) a first primer pair comprising a first F primer and a first R primer, wherein the first F primer comprises an amplification region binding region and a sequencing primer binding region;
- wherein the first R primer comprises a Library Tag binding region and a sequencing primer binding region;
- (d) a second primer pair comprising a second F primer and a second R primer, wherein each of the second F primer and the second R primer comprises an amplification region binding region and a sequencing primer binding region, wherein none of the primers of the second primer pair bind to the Library Tag.
2 more elements
Dependencies are inferred from the claim text.