Selective Capture of Target DNA Sequences
AssigneePersonal Genome Diagnostics Inc.
InventorJohn F. Thompson
Life of this patent
Estimating the term…
Abstract
Many regions of genomic DNA are highly similar to other regions of the genome and thus are very difficult to capture without also capturing the similar, undesired regions. This leads to over-sequencing of regions for which there is no interest and lowers coverage of the desired regions. To minimize the capture of non-desired regions, blocking baits have been designed to prevent similar but non-desired fragments from being captured. This allows more directed sequencing of the regions of interest. Blocking baits differ from capture baits in that they have modestly different sequence that preferentially bind the non-desired DNA and do not contain a biotin or other modification so remain behind when the capture baits are selected.
Claims20
A method of sequencing a target sequence comprising:
- a) hybridizing a sample nucleic acid with a capture nucleic acid molecule and a blocking nucleic acid molecule, wherein the sample nucleic acid comprises the target sequence and non-target sequence;
- b) isolating the capture nucleic acid molecule hybridized to the target sequence;
- and
- c) sequencing the isolated target sequence.
A method of improving sequencing specificity and/or accuracy of a target sequence comprising:
- a) hybridizing a sample nucleic acid with a capture nucleic acid molecule and a blocking nucleic acid molecule wherein the sample nucleic acid comprises the target sequence and non-target sequence;
- b) isolating the capture nucleic acid molecule hybridized to the target sequence;
- and
- c) sequencing the target sequence.
Dependencies are inferred from the claim text.