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US 2024/0191287 A1Published application
Modified biotin-binding proteins for immobilization
AssigneePacific Biosciences of California, Inc.
InventorsSatwik Kamtekar, Lubomir Sebo, Leewin Chern, Thomas Linsky, Jeremiah Hanes, Erik Miller, Ying Yang, Stephen Yue
Life of this patent
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Abstract
Compositions comprising covalently modified and mutated biotin-binding proteins, particularly biotin-binding proteins having a negative charge at physiological pH, are provided. Methods of producing such proteins are also provided, as are methods of immobilizing, sequencing, and making nucleic acids employing such proteins.
Claims23
1-95. (canceled)
A method of immobilizing a nucleic acid, the method comprising:
- providing a surface comprising a plurality of array regions, which array regions comprise biotin or a biotin analog;
- and
- exposing the surface to a complex comprising a nucleic acid and a biotin-binding protein, which biotin-binding protein has a calculated net charge of −20 or less at pH 7.4, whereby the biotin-binding protein binds to the biotin or biotin analog and thereby immobilizes the complex in the array regions.
(canceled)
117-154. (canceled)
Dependencies are inferred from the claim text.